ArticleApplied microbiology and biotechnology2022
Targeting intestinal flora and its metabolism to explore the laxative effects of rhubarb.
Article in Applied microbiology and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 58 citations in OpenAlex.
- Constipation Mitigation by Rhubarb Extract in Middle-Aged Adults Is Linked to Gut Microbiome Modulation: A Double-Blind Randomized Placebo-Controlled Trial.International journal of molecular sciences · 2022Trial
- Associations of probiotics combined with Tongli Gongxia Chinese medicine with intestinal barrier biomarkers and day 7 short-chain fatty acids in patients with severe acute pancreatitis.Frontiers in cellular and infection microbiology · 2026Article
- Active ingredients, nutrition values and health-promoting effects of aboveground parts of rhubarb: a review.Food science and biotechnology · 2026Review
- Comprehensive Review on the Toxicity of Five Main AQ Constituents from Rhubarb: Mechanisms, Challenges and Future Perspectives.Drug design, development and therapy · 2026Review
- Bioactive components of medicinal-food homology substances alleviating constipation via gut microbiota modulation: a review.Frontiers in pharmacology · 2026Review
- The Effects and Adverse Reactions of Different Bowel Cleansers on Gut Microbiota After Colonoscopy.The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology · 2025Review
- Rhubarb with Different Cooking Methods Restored the Gut Microbiota Dysbiosis and SCFAs in Ischemic Stroke Mice.Molecular neurobiology · 2025Article
- Bioactive Potential ofPlants (Basel, Switzerland) · 2025Article
- Exploring anti-SARS-CoV-2 natural products: dual-viral target inhibition by delphinidin and the anti-coronaviral efficacy of deapio platycodin D.Natural products and bioprospecting · 2025Article
- Emodin influences pyroptosis-related Caspase 1-GSDMD axis alleviated cerebral ischemia-reperfusion injury in rats.Scientific reports · 2025Article
- Runchangningshen paste activates NLRP6 inflammasome-mediated autophagy to stimulate colonic mucin-2 secretion and modulates mucosal microbiota in functional constipation.World journal of gastroenterology · 2025Article
- Green Tea with Rhubarb Root Reduces Plasma Lipids While Preserving Gut Microbial Stability in a Healthy Human Cohort.Metabolites · 2025Article
- Effects of dietary fiber on Chinese children with functional constipation and targeted modification of gut microbiota and related metabolites.Frontiers in nutrition · 2025Article
- The effects of Shenkang suppository on kidney function and gut microbiota in nondialysis patients with chronic kidney disease stages 3-4: A randomized controlled trial.Frontiers in pharmacology · 2025Article
- Segmented variable-frequency ultrasound synergistic hot-air drying of Rhubarb: Effect on drying characteristics and quality and thermal analysis.Ultrasonics sonochemistry · 2024Article
- Soluble Dietary Fiber of Hawthorn Relieves Constipation Induced by Loperamide Hydrochloride by Improving Intestinal Flora and Inflammation, Thereby Regulating the Aquaporin Ion Pathway in Mice.Foods (Basel, Switzerland) · 2024Article
- Taking SCFAs produced by Lactobacillus reuteri orally reshapes gut microbiota and elicits antitumor responses.Journal of nanobiotechnology · 2024Article
- Metabolomics for Clinical Biomarker Discovery and Therapeutic Target Identification.Molecules (Basel, Switzerland) · 2024Review
- The Water Extract of Rhubarb Prevents Ischemic Stroke by Regulating Gut Bacteria and Metabolic Pathways.Metabolites · 2024Article
- Effects of natural products on functional constipation: analysis of active ingredient and mechanism.Naunyn-Schmiedeberg's archives of pharmacology · 2024Review
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Authors and funding
11 authors at 1 institution in 1 country.
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Abstract
Rhubarb, a traditional herb, has been used in clinical practice for hundreds of years to cure constipation, but its mechanism is still not clear enough. Currently, growing evidence suggests that intestinal flora might be a potential target for the treatment of constipation. Thus, the aim of this study was to clarify the laxative effect of rhubarb via systematically analyzing the metagenome and metabolome of the gut microbiota. In this study, the laxative effects of rhubarb were investigated by loperamide-induced constipation in rats. The gut microbiota was determined by high-throughput sequencing of 16S rRNA gene. Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry was used for fecal metabolomics analysis. The data showed that rhubarb could significantly shorten gastrointestinal transit time, increase fecal water content and defecation frequency, improve gastrointestinal hormone disruption, and protect the colon mucus layer. Analysis of 16S rRNA gene sequencing indicated that rhubarb could improve the disorder of intestinal microbiota in constipated rats. For example, beneficial bacteria such as Ligilactobacillus, Limosilalactobacillus, and Prevotellaceae UCG-001 were remarkably increased, and pathogens such as Escherichia-Shigella were significantly decreased after rhubarb treatment. Additionally, the fecal metabolic profiles of constipated rats were improved by rhubarb. After rhubarb treatment, metabolites such as chenodeoxycholic acid, cholic acid, prostaglandin F2α, and α-linolenic acid were markedly increased in constipation rats; in contrast, the metabolites such as lithocholic acid, calcidiol, and 10-hydroxystearic acid were notably reduced in constipation rats. Moreover, correlation analysis indicated a close relationship between intestinal flora, fecal metabolites, and biochemical indices associated with constipation. In conclusion, the amelioration of rhubarb in constipation might modulate the intestinal microflora and its metabolism. Moreover, the application of fecal metabolomics could provide a new strategy to uncover the mechanism of herbal medicines.Key points• Rhubarb could significantly improve gut microbiota disorder in constipation rats.• Rhubarb could markedly modulate the fecal metabolite profile of constipated rats.
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