ArticleCritical care explorations2022
Inflammasome Activation in an In Vitro Sepsis Model Recapitulates Increased Monocyte Distribution Width Seen in Patients With Sepsis.
Article in Critical care explorations, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 13 citations in OpenAlex.
- Sepsis modelling: current approaches and organ-on-chip perspectives.EBioMedicine · 2026Review
- Sepsis Biomarkers in Evolution: Comparative Insights and the Promising Roles of MDW and Presepsin.Medicina (Kaunas, Lithuania) · 2026Review
- The Clinical and Economic Value of Incorporating Monocyte Distribution Width Testing in the Detection of Occult Sepsis.Journal of health economics and outcomes research · 2026Article
- Diagnostic Performance of Monocyte Distribution Width for the Detection of Sepsis: A Systematic Review and Meta-Analysis.Journal of the American College of Emergency Physicians open · 2025Review
- MONOCYTE ANISOCYTOSIS IS ASSOCIATED WITH SEPSIS IN CHILDREN WITH SUSPECTED INFECTION.Shock (Augusta, Ga.) · 2025Article
- Resolvin D2 restores monocyte anisocytosis and mediates a shift toward classical monocytes ex vivo in blood samples from patients after major burns.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Monocyte Anisocytosis Changes in Patients After Major Burn Injuries.Journal of burn care & research : official publication of the American Burn Association · 2025Article
- The Need for Standardized Guidelines for the Use of Monocyte Distribution Width (MDW) in the Early Diagnosis of Sepsis.Journal of personalized medicine · 2024Review
- Blood collection in heparin vs. EDTA results in an inflammasome-independent increase in monocyte distribution width at 4 h.Clinical chemistry and laboratory medicine · 2024Article
- Monocyte anisocytosis corresponds with increasing severity of COVID-19 in children.Frontiers in pediatrics · 2023Article
- Caspase-4 and -5 Biology in the Pathogenesis of Inflammatory Bowel Disease.Frontiers in pharmacology · 2022Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
objectivesIncreased monocyte distribution width (MDW) has recently been shown to be a reliable indicator of early sepsis detection. This study therefore sought to determine if inflammasome activation can be linked to monocyte size changes in sepsis.
designAn in vitro sepsis model using bacterial endotoxin (lipopolysaccharide [LPS]) to study the effect of inflammasome activation on monocyte cell size distribution by microscopy and MDW measurements using a standard clinical hematology analyzer.
settingUniversity research laboratory. SUBJECTS: Healthy adult volunteers and cultured human monocyte cells in wild-type state and after clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 knockout of key inflammasome components (apoptosis-associated speck-like protein containing a caspase recruitment domain, caspase-1, gasdermin-D).
interventionsIn vitro treatment of specimens with bacterial LPS. MEASUREMENTS AND MAIN
resultsWild-type THP1 cells demonstrated a significant increase in cell area (207 μm
conclusionsThe increased MDW seen in patients with sepsis can be reproduced in an in vitro sepsis model and blocked using clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 technology to inactivate the inflammasome. These findings suggest that pyroptotic cellular swelling underlies changes in MDW in septic patients and connect MDW to early events in the inflammatory cascade of sepsis.
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Registered trials
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