Evidence map›Paper›PMID 35127987›Full record

ArticleBio-protocol2022

Simple Scalable Protein Expression and Extraction Using Two-stage Autoinducible Cell Autolysis and DNA/RNA Autohydrolysis in

Romel Menacho-Melgar, Michael D Lynch

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 60% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Temperature-Dependent Recombinase-Based Genetic Circuits.International journal of molecular sciences · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Romel Menacho-MelgarDepartment of Biomedical Engineering, Duke University, Durham, NC, USA.
Michael D LynchDepartment of Biomedical Engineering, Duke University, Durham, NC, USA.
Duke University · US

Funding

Rapid diagnosis and quantification of HIV by direct capture, labelling and detection of individual virionsR61AI140485 · NIAID · DUKE UNIVERSITY · PI CHILKOTI, ASHUTOSH, LYNCH, MICHAEL DAVID · 2019 to 2021
$1.6M
NIAID NIH HHS R61 AI140485
6 · The paper itself

Abstract

Recombinant protein expression is extensively used in biological research. Despite this, current protein expression and extraction methods are not readily scalable or amenable for high-throughput applications. Optimization of protein expression conditions using traditional methods, reliant on growth-associated induction, is non-trivial. Similarly, protein extraction methods are predominantly restricted to chemical methods, and mechanical methods reliant on expensive specialized equipment more tuned for large-scale applications. In this article, we outline detailed protocols for the use of an engineered autolysis/autohydrolysis

Indexed as

AutoinductionAutolysisHigh-throughputPhosphate depletionProtein expressionScalabilityStationary phaseTwo-stage

Identifiers

PMID35127987
PMCPMC8799905
OpenAlexW4206618672

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.