ArticleFrontiers in public health2021
Metagenomic Next-Generation Sequencing vs. Traditional Microbiological Tests for Diagnosing Varicella-Zoster Virus Central Nervous System Infection.
Article in Frontiers in public health, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- Serious neurological adverse events in immunocompetent children and adolescents caused by viral reactivation in the years following varicella vaccination.Reviews in medical virology · 2024Pooled it
- Rashless varicella-zoster virus encephalitis diagnosed by metagenomic next-generation sequencing: two case reports.BMC neurology · 2026Article
- Varicella zoster virus-related myelitis: a case series and literature review.Virology journal · 2026Review
- Varicella Zoster Virus Infection: Clinical Features, Molecular Pathogenesis, Treatment, and Prevention.MedComm · 2026Review
- Toxoplasma gondii infection diagnosed by tNGS in a patient with acute lymphoblastic leukemia: a case report.BMC infectious diseases · 2025Article
- Optimizing metagenomic next-generation sequencing in CNS infections: a diagnostic model based on CSF parameters.Frontiers in cellular and infection microbiology · 2025Article
- Identification of Pathogens in HIV-Infected Patients Using Metagenomic Next-Generation Sequencing (mNGS) as Compared to Conventional Microbiological Tests (CMTs).Infection and drug resistance · 2025Article
- BALF metagenomic next-generation sequencing for the diagnosis of pulmonary mycobacterial infection in persons with HIV: a retrospective, diagnostic accuracy study.Frontiers in microbiology · 2025Article
- Exploring the appropriate situation of performing CSF mNGS in patients with proposed intracranial infections.BMC neurology · 2024Article
- Nanopore targeted sequencing-based diagnosis of central nervous system infections in HIV-infected patients.Annals of clinical microbiology and antimicrobials · 2024Article
- Metagenomic next-generation sequencing of cerebrospinal fluid: a diagnostic approach for varicella zoster virus-related encephalitis.Frontiers in cellular and infection microbiology · 2024Article
- Case Report: Taking action or standing by: managing a preterm neonate at the risk of neonatal varicella by metagenomic next-generation sequencing.Frontiers in pediatrics · 2024Article
- Advances in diagnosis and management of atypical spinal infections: A comprehensive review.North American Spine Society journal · 2023Article
- Clinical metagenomics-challenges and future prospects.Frontiers in microbiology · 2023Review
- Case report: A toxoplasmic encephalitis in an immunocompromised child detected through metagenomic next-generation sequencing.Frontiers in public health · 2023Article
- Clinical features of varicella-zoster virus caused neurological diseases detected by metagenomic next-generation sequencing.Open medicine (Warsaw, Poland) · 2023Article
- Metagenomic next-generation sequencing for identification of central nervous system pathogens in HIV-infected patients.Frontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundUnbiased metagenomic next-generation sequencing (mNGS) detects pathogens in a target-independent manner. It is not well-understood whether mNGS has comparable sensitivity to target-dependent nucleic acid test for pathogen identification.
methodsThis study included 31 patients with chickenpox and neurological symptoms for screening of possible varicella-zoster virus (VZV) central nervous system (CNS) infection. Microbiological diagnosing of VZV cerebrospinal fluid (CSF) infection was performed on stored CSF samples using mNGS, quantitative and qualitative VZV-specific PCR assays, and VZV IgM antibodies test.
resultsThe median age was 30.0 [interquartile range (IQR), 24.3-33.3] years. 51.6% of the patients were men. About 80.6% of the patients had normal CSF white blood cell counts (≤ 5 × 10
conclusionsOur study suggests that CSF mNGS may have higher sensitivity for VZV detection than CSF VZV PCR and antibody tests, and has the advantage of identifying unexpected pathogens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.