ArticleScience China. Chemistry2022
An automated nucleic acid detection platform using digital microfluidics with an optimized Cas12a system.
Article in Science China. Chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Carbene-catalytic enantioselective synthesis of chiral macrocycles.Chemical science · 2025Review
- Integrated Enzyme-Mediated One-Step Sample Processing and Duplex Amplification System for Rapid Detection ofFoods (Basel, Switzerland) · 2025Article
- Nucleic acid amplification tests in digital microfluidics: the promise of next-generation point-of-care diagnostics.Microsystems & nanoengineering · 2025Review
- Recent advances in organocatalytic atroposelective reactions.Beilstein journal of organic chemistry · 2025Review
- Structural Optimization of Microfluidic Chips for Enhancing Droplet Manipulation and Observation via Electrodynamics Simulation.Cyborg and bionic systems (Washington, D.C.) · 2025Article
- Rapid and portable quantification of HIV RNA via a smartphone-enabled digital CRISPR device and deep learning.Sensors and actuators reports · 2024Article
- Giant oligomeric porous cage-based molecules.Chemical science · 2024Article
- A photocontrolled one-pot isothermal amplification and CRISPR-Cas12a assay for rapid detection of SARS-CoV-2 Omicron variants.Microbiology spectrum · 2024Article
- Recent Advances in Field-Effect Transistor-Based Biosensors for Label-Free Detection of SARS-CoV-2.Small science · 2024Article
- Recent advancements of covalent organic frameworks (COFs) as proton conductors under anhydrous conditions for fuel cell applications.RSC advances · 2023Review
- CRISPR-Cas-based techniques for pathogen detection: Retrospect, recent advances, and future perspectives.Journal of advanced research · 2023Review
- Microfluidic-based technologies for diagnosis, prevention, and treatment of COVID-19: recent advances and future directions.Biomedical microdevices · 2023Review
- Microfluidics for COVID-19: From Current Work to Future Perspective.Biosensors · 2023Review
- Microfluidic chip and isothermal amplification technologies for the detection of pathogenic nucleic acid.Journal of biological engineering · 2022Review
- Fabrication of Transparent and Flexible Digital Microfluidics Devices.Micromachines · 2022Article
- Recent advances in recombinase polymerase amplification: Principle, advantages, disadvantages and applications.Frontiers in cellular and infection microbiology · 2022Review
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Outbreaks of both influenza virus and the novel coronavirus SARS-CoV-2 are serious threats to human health and life. It is very important to establish a rapid, accurate test with large-scale detection potential to prevent the further spread of the epidemic. An optimized RPA-Cas12a-based platform combined with digital microfluidics (DMF), the RCD platform, was established to achieve the automated, rapid detection of influenza viruses and SARS-CoV-2. The probe in the RPA-Cas12a system was optimized to produce maximal fluorescence to increase the amplification signal. The reaction droplets in the platform were all at the microliter level and the detection could be accomplished within 30 min due to the effective mixing of droplets by digital microfluidic technology. The whole process from amplification to recognition is completed in the chip, which reduces the risk of aerosol contamination. One chip can contain multiple detection reaction areas, offering the potential for customized detection. The RCD platform demonstrated a high level of sensitivity, specificity (no false positives or negatives), speed (≤30 min), automation and multiplexing. We also used the RCD platform to detect nucleic acids from influenza patients and COVID-19 patients. The results were consistent with the findings of qPCR. The RCD platform is a one-step, rapid, highly sensitive and specific method with the advantages of digital microfluidic technology, which circumvents the shortcomings of manual operation. The development of the RCD platform provides potential for the isothermal automatic detection of nucleic acids during epidemics. Electronic Supplementary Material: Supplementary material is available in the online version of this article at 10.1007/s11426-021-1169-1.
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