ArticleFrontiers in neurology2021
Restoring Function After Severe Spinal Cord Injury Through BioLuminescent-OptoGenetics.
Article in Frontiers in neurology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Who cites it
25 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Sex-dependent plasticity of adult neural tissue in response to damage.Development (Cambridge, England) · 2026Article
- CaBLAM: a high-contrast bioluminescent CaNature methods · 2026Article
- Control of Synaptic Communication through Molecularly Engineered Bioluminescence Light Emission and Sensing.bioRxiv : the preprint server for biology · 2025Article
- CaBLAM! A high-contrast bioluminescent CabioRxiv : the preprint server for biology · 2025Article
- Neuroplasticity and Nervous System Recovery: Cellular Mechanisms, Therapeutic Advances, and Future Prospects.Brain sciences · 2025Review
- Presymptomatic targeted circuit manipulation for ameliorating Huntington's disease pathogenesis.iScience · 2025Article
- Strength of activation and temporal dynamics of bioluminescent-optogenetics in response to systemic injections of the luciferin.NeuroImage · 2024Article
- Bioluminescent Systems for Theranostic Applications.International journal of molecular sciences · 2024Review
- Bioluminescence as a functional tool for visualizing and controlling neuronal activityNeurophotonics · 2024Article
- Efficient opto- and chemogenetic control in a single molecule driven by FRET-modified bioluminescence.Neurophotonics · 2024Article
- Method for optimizing imaging parameters to record neuronal and cellular activity at depth with bioluminescence.Neurophotonics · 2024Article
- Engineering luminopsins with improved coupling efficiencies.Neurophotonics · 2024Article
- BioLuminescent OptoGenetics in the choroid plexus: integrated opto- and chemogenetic controlNeurophotonics · 2024Article
- Recent advances in bioluminescent probes for neurobiology.Neurophotonics · 2024Article
- Photoactivation of LOV domains with chemiluminescence.Chemical science · 2024Article
- Engineering luminopsins with improved coupling efficiencies.bioRxiv : the preprint server for biology · 2023Article
- Optogenetic spinal stimulation promotes new axonal growth and skilled forelimb recovery in rats with sub-chronic cervical spinal cord injury.Journal of neural engineering · 2023Article
- Bioluminescent Genetically Encoded Glutamate Indicators for Molecular Imaging of Neuronal Activity.ACS synthetic biology · 2023Article
- Coelenterazine-Type Bioluminescence-Induced Optical Probes for Sensing and Controlling Biological Processes.International journal of molecular sciences · 2023Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to manipulate specific neuronal populations of the spinal cord following spinal cord injury (SCI) could prove highly beneficial for rehabilitation in patients through maintaining and strengthening still existing neuronal connections and/or facilitating the formation of new connections. A non-invasive and highly specific approach to neuronal stimulation is bioluminescent-optogenetics (BL-OG), where genetically expressed light emitting luciferases are tethered to light sensitive channelrhodopsins (luminopsins, LMO); neurons are activated by the addition of the luciferase substrate coelenterazine (CTZ). This approach utilizes ion channels for current conduction while activating the channels through the application of a small chemical compound, thus allowing non-invasive stimulation and recruitment of all targeted neurons. Rats were transduced in the lumbar spinal cord with AAV2/9 to express the excitatory LMO3 under control of a pan-neuronal or motor neuron-specific promoter. A day after contusion injury of the thoracic spine, rats received either CTZ or vehicle every other day for 2 weeks. Activation of either neuron population below the level of injury significantly improved locomotor recovery lasting beyond the treatment window. Utilizing histological and gene expression methods we identified neuronal plasticity as a likely mechanism underlying the functional recovery. These findings provide a foundation for a rational approach to spinal cord injury rehabilitation, thereby advancing approaches for functional recovery after SCI. SUMMARY: Bioluminescent optogenetic activation of spinal neurons results in accelerated and enhanced locomotor recovery after spinal cord injury in rats.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.