Evidence map›Paper›PMID 35126174›Full record

ArticleFrontiers in physiology2021

Acid-Sensing Ion Channel 1/Calpain1 Activation Impedes Macrophage ATP-Binding Cassette Protein A1-Mediated Cholesterol Efflux Induced by Extracellular Acidification.

Yuan-Mei Wang, Mo-Ye Tan, Rong-Jie Zhang, Ming-Yue Qiu, You-Sheng Fu, Xue-Jiao Xie, Hong-Feng Gu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
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  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Yuan-Mei WangHengyang Key Laboratory of Neurodegeneration and Cognitive Impairment and Institute of Neuroscience, Hengyang Medical College, University of South China, Hengyang, China.
Mo-Ye TanDepartment of Zhongjing Theory, College of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, China.
Rong-Jie ZhangHengyang Key Laboratory of Neurodegeneration and Cognitive Impairment and Institute of Neuroscience, Hengyang Medical College, University of South China, Hengyang, China.
Ming-Yue QiuHengyang Key Laboratory of Neurodegeneration and Cognitive Impairment and Institute of Neuroscience, Hengyang Medical College, University of South China, Hengyang, China.
You-Sheng FuHengyang Hospital of Traditional Chinese Medicine, Hengyang, China.
Xue-Jiao XieDepartment of Zhongjing Theory, College of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, China.
Hong-Feng GuHengyang Key Laboratory of Neurodegeneration and Cognitive Impairment and Institute of Neuroscience, Hengyang Medical College, University of South China, Hengyang, China.
University of South China · CNHunan University of Traditional Chinese Medicine · CNHengyang Academy of Agricultural Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundExtracellular acidification is a common feature of atherosclerotic lesions, and such an acidic microenvironment impedes ATP-binding cassette transporter A1 (ABCA1)-mediated cholesterol efflux and promotes atherogenesis. However, the underlying mechanism is still unclear. Acid-sensing ion channel 1 (ASIC1) is a critical H

aimIn this study, we explored whether or how ASIC1 influences extracellular acidification-induced ABCA1-mediated cholesterol efflux from macrophage-derived foam cells.

methodsRAW 264.7 macrophages were cultured in an acidic medium (pH 6.5) to generate foam cells. Then the intracellular lipid deposition, cholesterol efflux, and ASIC1/calpain1/ABCA1 expressions were evaluated.

resultsWe showed that extracellular acidification enhanced ASIC1 expression and translocation, promoted calpain1 expression and lipid accumulation, and decreased ABCA1 protein expression as well as ABCA1-mediated cholesterol efflux. Of note, inhibiting ASIC1 activation with amiloride or Psalmotoxin 1 (PcTx-1) not only lowered calpain1 protein level and lipid accumulation but also enhanced ABCA1 protein levels and ABCA1-mediated cholesterol efflux of macrophages under extracellular acidification conditions. Furthermore, similar results were observed in macrophages treated with calpain1 inhibitor PD150606.

conclusionExtracellular acidification declines cholesterol efflux

Indexed as

ABCA1ASIC1calpain1cholesterol effluxextracellular acidificationfoam cells

Identifiers

PMID35126174
PMCPMC8811198
OpenAlexW4205260227

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.