Evidence map›Paper›PMID 35125885›Full record

ArticleInternational journal of general medicine2022

eQTL Highlights the Potential Role of Negative Control of Innate Immunity in Kawasaki Disease.

Sirui Song, Liqin Chen, Qianqian Ning, Danying Zhu, Feng Qiu, Guang Li, Hong Zhang, Tingting Xiao, Guohui Ding, Min Huang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of general medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 99% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Sirui SongDepartment of Cardiology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Liqin ChenDepartment of Cardiology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Qianqian NingShanghai Center for Bioinformation Technology, Shanghai, People's Republic of China.
Danying ZhuDepartment of Cardiology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Feng QiuShanghai Center for Bioinformation Technology, Shanghai, People's Republic of China.
Guang LiShanghai Center for Bioinformation Technology, Shanghai, People's Republic of China.
Hong ZhangDepartment of Laboratory, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Tingting XiaoDepartment of Cardiology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Guohui DingInternational Human Phenome Institutes, Shanghai, 200235, People's Republic of China.
Min HuangDepartment of Cardiology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Shanghai Center For Bioinformation Technology · CNShanghai Institutes for International Studies · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeKawasaki disease (KD) is an acute systemic vasculitis mainly found in the medium-sized arteries, especially the coronary arteries. Immune system is involved in the pathogenesis of acute KD in children, but the functional differences in the immune system between healthy children and KD patients remain unclear. PATIENTS AND

methodsA total of 190 KD patients and 119 healthy controls were recruited for the next-generation sequencing of 512 targeted genes from 4 immune-related pathways. Subsequently, the peripheral blood mononuclear cells (PBMCs) were isolated. RNA sequencing of the LPS treated PBMCs from additional 20 KD patients and 20 healthy controls was used to examine the differentially expressed genes (DEGs). Then, an expression quantitative trait locus (eQTL) analysis combined with previously analyzed RNA data were used to examine the DEGs. Finally, the serum levels of 13 cytokines were detected before and after LPS treatment in 40 samples to confirm the findings from eQTL analysis.

resultsA total of 319 significant eQTL were found, and both eQTL analysis and RNA sequencing showed some DEGs were involved in the connective tissue disorders and inflammatory diseases. DEGs that function to negatively regulate immunity were closely related to the pathogenesis of KD. In addition, the serum levels of IL-10 (an inflammatory and immunosuppressive factor) and SCD25 (an important immunosuppressant) reduced significantly in the KD patients.

conclusionOur study shows the expression of factors responsible for the negative control of innate immunity is altered, which plays an important role in the etiology of KD.

Indexed as

cytokinesinflammationinnate immunityKawasaki diseasenext generation sequencing

Identifiers

PMID35125885
PMCPMC8807868
OpenAlexW4206954482

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.