Evidence map›Paper›PMID 35122574›Full record

ArticleMarine biotechnology (New York, N.Y.)2022

Sex-Specific Genomic Region Identification and Molecular Sex Marker Development of Rock Bream (Oplegnathus fasciatus).

Jie Gong, Bijun Li, Ji Zhao, Zhixiong Zhou, Qiaozhen Ke, Qihui Zhu, Dongdong Xu, Tao Zhou, Peng Xu

Abstract read
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In one paragraph

Article in Marine biotechnology (New York, N.Y.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jie Gong *Fujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, China.
Bijun Li *Fujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, China.
Ji ZhaoFujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, China.
Zhixiong ZhouFujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, China.
Qiaozhen KeFujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, China.
Qihui ZhuZhejiang Marine Fisheries Research Institute, Zhoushan, China.
Dongdong XuZhejiang Marine Fisheries Research Institute, Zhoushan, China.
Tao ZhouFujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, China.
Peng XuFujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, China. xupeng77@xmu.edu.cn.ORCID http://orcid.org/0000-0002-1531-5078

Funding

China Agriculture Research System CARS-47National Key R&D Program of China 2019YFE0119000Ningbo Science and Technology Innovation 2025 Major Project 2021Z002Special Foundation for Major Research Program of Fujian Province 2020NZ08003the special project of local science and technology development guided by the central government 2019L3032Zhejiang Provincial Natural Science Foundation of China LQ20C190008
6 · The paper itself

Abstract

Rock bream (Oplegnathus fasciatus) is a valuable commercial marine teleost species, which exhibits sexual dimorphism in growth performance. However, the absence of a rapid and cost-effective sex identification method based on sex-specific genetic marker has impeded study on sex determination mechanisms and breeding applications. In the present study, we firstly developed the PCR method for identifying potential sex-specific sequences in Oplegnathus fasciatus with the next-generation sequencing. Sex-specific genomic regions/loci for sex determination were discovered on Chr2 and Chr6 by genome-wide association analysis, sequencing depth, and heterozygosity comparison between females and males. Candidate sex-determining genes (CCDC63, ITR, WNT4) were furtherly detected in transcriptome data of testes and ovaries. Taken together, a male-specific 34-bp deletion on the Chr2 was identified and developed into molecular marker of sex for O. fasciatus. After validation in individuals with known phenotypic sexes, the accuracy was 100%. This study gives an insight into the mechanism of sex determination in O. fasciatus, and the gender marker is crucial both for future genomic research and for development of efficient and sustainable aquaculture practice.

Indexed as

Genome-Wide Association StudyPerciformesAnimalsBase SequenceFemaleFish ProteinsGenomicsHumansMalePhylogenyFish ProteinsGenetic sex identificationGenomic resourcesOplegnathus fasciatusSex determination systemSex molecular marker

Identifiers

PMID35122574

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