Evidence map›Paper›PMID 35121076›Full record

ArticleMolecular and cellular endocrinology2022

Deletion of the homeodomain gene Six3 from kisspeptin neurons causes subfertility in female mice.

Shanna N Lavalle, Teresa Chou, Jacqueline Hernandez, Nay Chi P Naing, Michelle Y He, Karen J Tonsfeldt, Pamela L Mellon

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and cellular endocrinology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Shanna N LavalleDepartment of Obstetrics, Gynecology, And Reproductive Sciences, Center for Reproductive Science and Medicine, University of California, San Diego, La Jolla, CA, 92093, USA. Electronic address: snewton@health.ucsd.edu.
Teresa ChouDepartment of Obstetrics, Gynecology, And Reproductive Sciences, Center for Reproductive Science and Medicine, University of California, San Diego, La Jolla, CA, 92093, USA. Electronic address: teresa.chou@northwestern.edu.
Jacqueline HernandezDepartment of Obstetrics, Gynecology, And Reproductive Sciences, Center for Reproductive Science and Medicine, University of California, San Diego, La Jolla, CA, 92093, USA. Electronic address: jah151@ucsd.edu.
Nay Chi P NaingDepartment of Obstetrics, Gynecology, And Reproductive Sciences, Center for Reproductive Science and Medicine, University of California, San Diego, La Jolla, CA, 92093, USA. Electronic address: nnaing@ucsd.edu.
Michelle Y HeDepartment of Obstetrics, Gynecology, And Reproductive Sciences, Center for Reproductive Science and Medicine, University of California, San Diego, La Jolla, CA, 92093, USA. Electronic address: m3he@ucsd.edu.
Karen J TonsfeldtDepartment of Obstetrics, Gynecology, And Reproductive Sciences, Center for Reproductive Science and Medicine, University of California, San Diego, La Jolla, CA, 92093, USA. Electronic address: ktonsfeldt@health.ucsd.edu.
Pamela L MellonDepartment of Obstetrics, Gynecology, And Reproductive Sciences, Center for Reproductive Science and Medicine, University of California, San Diego, La Jolla, CA, 92093, USA. Electronic address: pmellon@health.ucsd.edu.
University of California San Diego · USUC San Diego Health System · US

Funding

VIRAL MALIGNANCYP30CA023100 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI DIANE M SIMEONE · 1985 to 2026
$124.9M
Yeast Genetics and Stress Response Genes-BiomedicalP42ES010337 · NIEHS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI TUKEY, ROBERT H · 2000 to 2022
$69.8M
Transgenic & Knock-out MouseP30DK063491 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI PETER J TONTONOZ · 2003 to 2026
$40.4M
Training Program in Basic Clinical GeneticsT32GM008666 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HAMILTON, BRUCE A · 1998 to 2021
$8.6M
Testosterone-GnRH Frequency and the Evolution of PCOS in AdolescenceP50HD028934 · NICHD · UNIVERSITY OF VIRGINIA · PI HAISENLEDER, DANIEL J. · 2014 to 2018
$8.6M
The Role of FSH in Female InfertilityP50HD012303 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI MELLON, PAMELA L · 1985 to 2020
$7.5M
TRAINING IN REPRODUCTIVE SCIENCEST32HD007203 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI PAMELA L MELLON · 1988 to 2026
$4.8M
NEUROSECRETORY GENE EXPRESSION IN THE HYPOTHALAMUSR01DK044838 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI MELLON, PAMELA L · 1991 to 2011
$3.8M
Neurosecretory Gene Expression in the HypothalamusR01HD100580 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI MELLON, PAMELA L · 2020 to 2024
$2.7M
CRR LIGAND ASSAY AND ANALYSIS CORER24HD102061 · NICHD · UNIVERSITY OF VIRGINIA · PI HAISENLEDER, DANIEL J. · 2019 to 2023
$2.2M
Neurosecretory Gene Expression in the HypothalamusR01HD072754 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI MELLON, PAMELA L · 2012 to 2016
$1.7M
Neuroendocrine Actions of Androgens in Female ReproductionR01HD082567 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI MELLON, PAMELA L · 2015 to 2019
$1.6M
NCI NIH HHS P30 CA023100NICHD NIH HHS F32 HD090837NICHD NIH HHS P50 HD012303NICHD NIH HHS P50 HD028934NICHD NIH HHS R01 HD072754NICHD NIH HHS R01 HD082567NICHD NIH HHS R01 HD100580NICHD NIH HHS R24 HD102061NICHD NIH HHS T32 HD007203NIDDK NIH HHS P30 DK063491NIDDK NIH HHS R01 DK044838NIEHS NIH HHS P42 ES010337NIGMS NIH HHS T32 GM008666
6 · The paper itself

Abstract

The homeodomain transcription factor SIX3 is a known regulator of eye, nose, and forebrain development, and has recently been implicated in female reproduction. Germline heterozygosity of SIX3 is sufficient to cause subfertility, but the cell populations that mediate this role are unknown. The neuropeptide kisspeptin is a critical component of the reproductive axis and plays roles in sexual maturation, ovulation, and the maintenance of gonadotropin secretion. We used Cre-Lox technology to remove Six3 specifically from kisspeptin neurons in mice to test the hypothesis that SIX3 in kisspeptin neurons is required for reproduction. We found that loss of Six3 in kisspeptin neurons causes subfertility and estrous cycle irregularities in females, but no effect in males. Overall, we find that SIX3 expression in kisspeptin neurons is an important contributor to female fertility.

Indexed as

Eye ProteinsHomeodomain ProteinsInfertilityKisspeptinsNerve Tissue ProteinsNeuronsAnimalsFemaleGonadotropin-Releasing HormoneHomeobox Protein SIX3MaleMiceNeuropeptidesReproductionEye ProteinsGonadotropin-Releasing HormoneHomeobox Protein SIX3Homeodomain ProteinsKisspeptinsNerve Tissue ProteinsNeuropeptidesCre/LoxPFertilityHypothalamusKisspeptinSix3

Identifiers

PMID35121076
PMCPMC8934285
OpenAlexW4210414566

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.