ArticleNeuron2022
High-performance microbial opsins for spatially and temporally precise perturbations of large neuronal networks.
Article in Neuron, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 96 citations in OpenAlex.
- Crosstalk-free optical indicator-actuator combinations for bidirectional optogenetics.npj biomedical innovations · 2026Review
- The Importance of Synchrony in the Neural Control of Movement.bioRxiv : the preprint server for biology · 2026Article
- Tunable Bessel beam two-photon fluorescence microscopy for high-speed volumetric imaging of brain dynamics.eLife · 2026Article
- Multi-site temporal control of optogenetic stimulation enhances firing frequencies in peripheral nerves.bioRxiv : the preprint server for biology · 2026Article
- Feature-tuned synaptic inputs to somatostatin interneurons drive context-dependent processing.Neuron · 2026Article
- Computational optimization of two-photon holographic stimulation sitesJournal of neural engineering · 2026Article
- 2P-FENDO-II: A fiber bundle microscope for all-optical, large field-of-view brain studies in freely moving mice.Cell reports methods · 2026Article
- A2-pancortins interact with Bcl-xL and WAVE1 to promote mitochondria-ER contact sites (MERCs) and exacerbate mitochondrial calcium elevation to mediate cell death in stroke.Scientific reports · 2026Article
- Active pixel power control for crosstalk-free all-optical neural interrogation.Nature communications · 2026Article
- Bridging Model and Experiment in Systems Neuroscience with Cleo: The Closed-Loop, Electrophysiology, and Optophysiology Simulation Testbed.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Tunable Bessel beam two-photon fluorescence microscopy for high-speed volumetric imaging of brain dynamics.bioRxiv : the preprint server for biology · 2026Article
- Patterned wireless transcranial optogenetics generates artificial perception.Nature neuroscience · 2026Article
- Desensitization of opsin responses during all-optical interrogation depends on imaging parameters.Neurophotonics · 2026Article
- Technical development of two-photon optogenetic stimulation and its potential application to brain-machine interfaces.Neurophotonics · 2026Review
- Article
- Red-shifted excitation enhances the sensitivity of red genetically encoded CaJournal of biomedical optics · 2025Article
- Recurrent pattern completion drives the neocortical representation of sensory inference.Nature neuroscience · 2025Article
- Rapid learning of neural circuitry from holographic ensemble stimulation enabled by model-based compressed sensing.Nature neuroscience · 2025Article
- Rapid rebalancing of co-tuned ensemble activity in the auditory cortex.bioRxiv : the preprint server for biology · 2025Article
- Bridging model and experiment in systems neuroscience with Cleo: the Closed-Loop, Electrophysiology, and Optophysiology simulation testbed.bioRxiv : the preprint server for biology · 2025Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
Abstract
The biophysical properties of existing optogenetic tools constrain the scale, speed, and fidelity of precise optogenetic control. Here, we use structure-guided mutagenesis to engineer opsins that exhibit very high potency while retaining fast kinetics. These new opsins enable large-scale, temporally and spatially precise control of population neural activity. We extensively benchmark these new opsins against existing optogenetic tools and provide a detailed biophysical characterization of a diverse family of opsins under two-photon illumination. This establishes a resource for matching the optimal opsin to the goals and constraints of patterned optogenetics experiments. Finally, by combining these new opsins with optimized procedures for holographic photostimulation, we demonstrate the simultaneous coactivation of several hundred spatially defined neurons with a single hologram and nearly double that number by temporally interleaving holograms at fast rates. These newly engineered opsins substantially extend the capabilities of patterned illumination optogenetic paradigms for addressing neural circuits and behavior.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.