Evidence map›Paper›PMID 35120274›Full record

ArticleHepatology research : the official journal of the Japan Society of Hepatology2022

Perturbation of Wnt/β-catenin signaling and sexual dimorphism in non-alcoholic fatty liver disease.

Matthew M Yeh, Xiuhui Shi, Jingxuan Yang, Min Li, Kar-Ming Fung, Sayed S Daoud

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Hepatology research : the official journal of the Japan Society of Hepatology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Matthew M YehDepartment of Laboratory Medicine and Pathology, University of Washington, School of Medicine, Seattle, Washington, USA.ORCID https://orcid.org/0000-0002-3004-8799
Xiuhui ShiDepartment of Medicine and Department of Surgery, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA.ORCID https://orcid.org/0000-0002-5327-8247
Jingxuan YangDepartment of Medicine and Department of Surgery, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA.ORCID https://orcid.org/0000-0003-3265-161X
Min LiDepartment of Medicine and Department of Surgery, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA.ORCID https://orcid.org/0000-0002-3971-9130
Kar-Ming FungDepartment of Pathology and Stephenson Cancer Center, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA.ORCID https://orcid.org/0000-0002-4623-9469
Sayed S DaoudDepartment of Pharmaceutical Sciences, Washington State University Health Sciences, Spokane, Washington, USA.ORCID https://orcid.org/0000-0003-0757-8166
University of Oklahoma Health Sciences Center · USUniversity of Washington · USWashington State University Spokane · US

Funding

Tissue Pathology Shared ResourceP30CA225520 · NCI · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI Wade Williams · 2018 to 2026
$27.1M
NCI NIH HHS P30 CA225520
6 · The paper itself

Abstract

aimsThe prevalence of non-alcoholic fatty liver disease (NAFLD) and its progression to non-alcoholic steatohepatitis (NASH) is higher in postmenopausal women than men. The aim of this study was to determine the molecular mechanisms underlying this sexual dimorphism in NAFLD.

methodsA total of 24 frozen liver samples of both sexes (normal and NAFLD/NASH) were used in this study. Total RNAseq was first used to identify differentially expressed genes (DEGs) between samples. Enrichment analysis of Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Reactome were used to analyze biological pathways. RT

resultsWe identified 4362 genes that are differentially expressed between NAFLD/NASH and normal samples; of those 745 genes were characterized as sex specific in NAFLD/NASH. Multiple pathway analysis platforms showed that Wnt-signaling is a candidate shared for a common biological pathway-associated with NAFLD/NASH. Using Wnt pathway focused PCR array we identified many genes involved in canonical pathway (Wnt/β-catenin activation) such as CTNNB1, c-Myc and CCND2 are overexpressed in female cases, whereas these genes are either not detected or downregulated in male cases. Immunoblot analysis validated the expression of CTNNB1 in female cases but not in male protein samples.

conclusionsOur study suggests, for the first time, that the activation of canonical Wnt signaling could be one of the main pathways associated with sexual dimorphism in NAFLD and NASH.

Indexed as

non-alcoholic fatty liver diseasenon-alcoholic steatohepatitisRNA-seqRT2-profiler PCR arrayssexual dimorphismWnt/β-catenin

Identifiers

PMID35120274
PMCPMC10874498
OpenAlexW4210709178

What OpenQuestion holds

Textmetadata
LicenceTDM
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.