Evidence map›Paper›PMID 35119684›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2022

Genetic diversity of Chlamydia pecorum detected in sheep flocks from Mexico.

M M Limón-González, R Hernández-Castro, F Martínez-Hernández, J Xicohtencatl-Cortes, H Ramírez-Alvarez, E G Palomares-Resendiz, E Díaz-Aparicio

Open access · greenAbstract read
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Dominance of Vaccine-SpecificEcology and evolution · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

M M Limón-GonzálezFacultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Coyoacán, 04510, Ciudad de México, Mexico. mwmagda@hotmail.com.
R Hernández-CastroDepartamento de Ecología de Agentes Patógenos, Hospital General "Dr. Manuel Gea González", Tlalpan, 14080, Ciudad de México, Mexico.
F Martínez-HernándezDepartamento de Ecología de Agentes Patógenos, Hospital General "Dr. Manuel Gea González", Tlalpan, 14080, Ciudad de México, Mexico.
J Xicohtencatl-CortesLaboratorio de Bacteriología Intestinal, Hospital Infantil de México "Dr. Federico Gómez", Cuauhtémoc, 06720, Ciudad de México, Mexico.
H Ramírez-AlvarezFacultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, 54714, Cuautitlán Izcalli, Estado de México, Mexico.
E G Palomares-ResendizCentro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad (CENID-SAI), Instituto Nacional de Investigaciones ForestalesAgrícolas y Pecuarias, Cuajimalpa, 05110, Ciudad de México, Mexico.
E Díaz-AparicioCentro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad (CENID-SAI), Instituto Nacional de Investigaciones ForestalesAgrícolas y Pecuarias, Cuajimalpa, 05110, Ciudad de México, Mexico.
Hospital General Dr. Manuel Gea Gonzalez · MXInstituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias · MXUniversidad Nacional Autónoma de México · MXHospital Infantil de México Federico Gómez · MX

Funding

SAGARPA CONACyT 2017-2-291311SEP-CONACYT-ANUIES-ECOS Francia 291241
6 · The paper itself

Abstract

Chlamydia pecorum, an obligate intracellular bacterium, is associated with reproductive and systemic diseases in sheep, goats, pigs, cattle, and koalas. The main conditions include polyarthritis, conjunctivitis, enteritis, pneumonia, encephalomyelitis, orchitis, placentitis, and abortion. Even though there are several studies showing that C. pecorum infections are widely spread in the world, in Mexico there are no reports. During 2016, as part of a sheep restocking program in Mexico, sheep were imported from New Zealand. Briefly after their arrival in the herds in the State of Mexico, these sheep presented abortions during the last third of gestation. A total of 62 sheep vaginal swabs that had presented abortion from different municipalities of the State of Mexico were collected. Bacterial isolation was performed using L929 mouse fibroblasts, and molecular identification was achieved by 23S rRNA (Chlamydiaceae family) and ompA gene (species-specific) real-time polymerase chain reaction (PCR). In addition, the 16S rRNA subunit and ompA gene were amplified and sequenced. Seven of 62 samples were positive for C. pecorum by bacterial isolation, 23S rRNA, and ompA gene real-time PCR. The 16S rRNA subunit and ompA gene amplicons were purified and the nucleotide sequence was determined in both directions. The consensus sequences homology search was performed using BLASTn analysis and showed a 100% of homology with the C. pecorum 16S rRNA subunit and 99% with the C. pecorum ompA gene. The population structure analyses using ompA gene demonstrated 15 genetic populations or clusters of 198 sequences from GenBank and our sequences were in a particular genetic structure corresponding to genotype "O." Herein, we describe the presence of C. pecorum in sheep imported from New Zealand into Mexico. Genetic analysis of the ompA gene showed that the isolates belong to genotype O and are related to strains isolated from sheep, cattle, and koalas.

Indexed as

Chlamydia InfectionsPhascolarctidaeSheep DiseasesAnimalsCattleChlamydiaFemaleGenetic VariationMaleMexicoMicePregnancyRNA, Ribosomal, 16SRNA, Ribosomal, 23SSheepSwineRNA, Ribosomal, 16SRNA, Ribosomal, 23S16S rRNAChlamydia pecorumompAPhylogenetic analysesSheep

Identifiers

PMID35119684
PMCPMC9151965
OpenAlexW4210697969

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.