ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2022
Under-Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle-Based Approach for Microscale Cell Culture.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Exclusive liquid repellency isolation (ELRi) microliter scale isolation technique unmasks T-cell migration defects to CCL21 in pediatric asthma.bioRxiv : the preprint server for biology · 2026Article
- In Vitro Monitoring of Babesia microti Infection Dynamics in Whole Blood Microenvironments.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Under-oil neuronal cell culture: Enhanced system stability, yield, and modulated oxygen environment.Journal of neuroscience methods · 2025Article
- PDMS SlipChip: Optimizing Sealing, Slipping, and Biocompatibility Using Low-Viscosity Silicone Oils.Micromachines · 2025Article
- Combining Top-Down and Bottom-Up: An Open Microfluidic Microtumor Model for Investigating Tumor Cell-ECM Interaction and Anti-Metastasis.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Article
- Visualization-enhanced under-oil open microfluidic system for in situ characterization of multi-phase chemical reactions.Nature communications · 2024Article
- The effect of whole blood logistics on neutrophil non-specific activation and kinetics ex vivo.Scientific reports · 2024Article
- The use of droplet-based microfluidic technologies for accelerated selection ofBiology methods & protocols · 2024Review
- Miniaturizing chemistry and biology using droplets in open systems.Nature reviews. Chemistry · 2023Review
- Under-oil open microfluidic systems for rapid phenotypic antimicrobial susceptibility testing.Lab on a chip · 2023Article
- Under-Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle-Based Approach for Microscale Cell Culture.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
Corrections and comments
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Authors and funding
15 authors.
Funding
Abstract
Oxygen levels in vivo are autonomously regulated by a supply-demand balance, which can be altered in disease states. However, the oxygen levels of in vitro cell culture systems, particularly microscale cell culture, are typically dominated by either supply or demand. Further, the oxygen microenvironment in these systems is rarely monitored or reported. Here, a method to establish and dynamically monitor autonomously regulated oxygen microenvironments (AROM) using an oil overlay in an open microscale cell culture system is presented. Using this method, the oxygen microenvironment is dynamically regulated via the supply-demand balance of the system. Numerical simulation and experimental validation of oxygen transport within multi-liquid-phase, microscale culture systems involving a variety of cell types, including mammalian, fungal, and bacterial cells are presented. Finally, AROM is applied to establish a coculture between cells with disparate oxygen demands-primary intestinal epithelial cells (oxygen consuming) and Bacteroides uniformis (an anaerobic species prevalent in the human gut).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.