Evidence map›Paper›PMID 35118425›Full record

ArticleSTAR protocols2022

A 2D and 3D cell culture protocol to study O-GlcNAc in sphingosine-1-phosphate mediated fibroblast contraction.

Murielle M Morales, Nichole J Pedowitz, Matthew R Pratt

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 60% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Murielle M MoralesBiological Sciences, University of Southern California, Los Angeles, CA 90089, USA.
Nichole J PedowitzDepartments of Chemistry, University of Southern California, Los Angeles, CA 90089, USA.
Matthew R PrattBiological Sciences, University of Southern California, Los Angeles, CA 90089, USA.
University of Southern California · US

Funding

Chemical Tools for the Investigation and Manipulation of Protein GlycosylationR01GM125939 · NIGMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI PRATT, MATTHEW ROBERT · 2017 to 2025
$2.4M
CBI: Chemistry Biology InterfaceT32GM118289 · NIGMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI FORSBURG, SUSAN L · 2016 to 2020
$1.2M
NIGMS NIH HHS R01 GM125939NIGMS NIH HHS T32 GM118289
6 · The paper itself

Abstract

O-linked β-N-acetylglucosamine (O-GlcNAc) is an abundant posttranslational modification involved in a wide range of signaling pathways, but its specific role in regulating biological processes remains unclear. This protocol describes approaches to understand O-GlcNAc's role in fibroblast contraction. Specifically, cellular O-GlcNAc levels are controlled through treatment of fibroblasts with inhibitors in both 2D and 3D cultures. We then describe 2D contraction assay and 3D collagen gel contraction assay to analyze the effect of the modification on sphingosine-1-phosphate signaling for contraction. For complete details on the use and execution of this protocol, please refer to Pedowitz et al. (2021).

Indexed as

AcetylglucosamineAnimalsCell Culture TechniquesFibroblastsHumansLysophospholipidsMiceNIH 3T3 CellsProtein Processing, Post-TranslationalSignal TransductionSphingosineAcetylglucosamineLysophospholipidsSphingosinesphingosine 1-phosphateBiophysicsCell-based AssaysCell BiologyCell cultureMicroscopyMolecular/Chemical Probes

Identifiers

PMID35118425
PMCPMC8792430
OpenAlexW4206307116

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.