ArticleReproduction & fertility2021
Sperm morphology and DNA fragmentation after zona pellucida selection.
Article in Reproduction & fertility, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 12 citations in OpenAlex.
- Use of Artificial Intelligence in the choice of sperm for ICSI - A Literature Review.JBRA assisted reproduction · 2026Pooled it
- Sperm morphology indices as exploratory screening tools for elevated DNA fragmentation: a single-centre correlation and threshold derivation study.BMC urology · 2026Article
- Sperm Testing in Assisted Reproductive Technology: Diagnosis and Outcome Prediction.Reproductive sciences (Thousand Oaks, Calif.) · 2026Review
- Sperm preparation techniques affect biological parameters, fertility indices, and DNA fragmentation in patients with oligo-astheno-teratozoospermia.Reproduction & fertility · 2025Article
- Improving sperm selection strategies for assisted reproduction through closing the knowledge gap in sperm maturation mechanics.Human reproduction open · 2025Review
- Paternal age, de novo mutations, and offspring health? New directions for an ageing problem.Human reproduction (Oxford, England) · 2024Review
- A narrative review of sperm selection technology for assisted reproduction techniques.Translational andrology and urology · 2024Review
- Cumulus matrix selection leads to isolation of spermatozoa with better motility, morphology, and lower DNA fragmentation.Reproduction & fertility · 2024Article
- New horizons in human sperm selection for assisted reproduction.Frontiers in endocrinology · 2023Review
- Human Blastocyst Components Detection Using Multiscale Aggregation Semantic Segmentation Network for Embryonic Analysis.Biomedicines · 2022Article
- Sperm morphology and DNA fragmentation after zona pellucida selection.Reproduction & fertility · 2021Article
- Sperm Selection Using Zona Pellucida-Binding Enhanced Embryo Morphokinetic and Clinical Outcomes in ICSI: A Sibling Oocytes Study.American journal of men's healthArticle
- Assisted Sperm Fusion Insemination: A Fusion-Based Approach to Fertilization Without Mechanical Oolemma Penetration.Reproductive medicine and biologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sperm DNA fragmentation (SDF) and sperm morphological defects can negatively affect ART outcomes. Consequently, there is a need for additional semen processing technique that accounts for sperm DNA status and morphology prior to ICSI. The objective was to evaluate the efficacy of an additional zona pellucida adhesion-based sperm selection for obtaining sperm populations with a high percentage of normal morphology and DNA integrity as compared to native semen and routine swim-up preparation. Semen samples from 78 normozoospermic men were subjected to swim up and placed in petri dishes coated with 48 acid-solubilized zonae pellucidae. Sperm DNA fragmentation and morphology were assessed in the native semen, the swim-up samples, and the zona-adhered spermatozoa from each patient. The mean sperm DNA fragmentation of the zona-selected spermatozoa (3.5 ± 0.7%) was significantly lower than the swim-up samples (15.3 ± 5.2%) ( LAY SUMMARY: High level of DNA folding known as sperm DNA fragmentation (SDF) inside each sperm and defects in the shape, size, and structure of the sperm can negatively affect assisted reproduction treatment (ART) outcomes. Consequently, there is a need for additional semen processing techniques that account for sperm quality prior to ART. Our team designed a simple technique using proteins from the coat around the egg (zona pellucida) to enhance sperm selection procedures based on natural sperm-egg interactions. Using this technique in combination with the most common techniques used in ART yields a significantly higher percentage of sperm with normal shape, size, and structure and a decreased level of DNA fragmentation. This sperm zona-selection technique would be beneficial if introduced in the ART practice to yield sperm with higher fertilization potential.
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Registered trials
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