Evidence map›Paper›PMID 35118177›Full record

ArticleBio-protocol2022

Identification of Protein-RNA Interactions in Mouse Testis Tissue Using fRIP.

Alexis S Bailey, Pedro J Batista, Howard Y Chang, Margaret T Fuller

Open access · hybridAbstract read
In one paragraph

Article in Bio-protocol, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 60% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Alexis S BaileyDepartment of Developmental Biology, Stanford University School of Medicine, Stanford, United States.
Pedro J BatistaLaboratory of Cell Biology, Center for Cancer Research, National Cancer Institute (NIH), Bethesda, United States.
Howard Y ChangCenter for Personal Dynamic Regulomes, Stanford University School of Medicine, Stanford, United States.
Margaret T FullerDepartment of Developmental Biology, Stanford University School of Medicine, Stanford, United States.
Stanford University · US

Funding

Regulation of RNA biogenesis and function by RNA modificationsZIABC011766 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI DE OLIVEIRA RODRIGUES BATISTA, PEDRO · 2017 to 2025
$10.6M
Regulation of proliferation and differentiation in the male germ line adult stem cell lineageR35GM136433 · NIGMS · STANFORD UNIVERSITY · PI MARGARET T FULLER · 2020 to 2026
$5.9M
Postgraduate Training Program in Epithelial BiologyT32AR007422 · NIAMS · STANFORD UNIVERSITY · PI PAUL KHAVARI · 1986 to 2026
$5.0M
Structural Motifs in RNAR01HG004361 · NHGRI · STANFORD UNIVERSITY · PI CHANG, HOWARD Y · 2007 to 2018
$4.7M
Conserved regulation of the switch from proliferation to differentiation in the germ line stem cell lineageR01GM122951 · NIGMS · STANFORD UNIVERSITY · PI FULLER, MARGARET T · 2018 to 2020
$956k
NHGRI NIH HHS R01 HG004361NIAMS NIH HHS T32 AR007422NIGMS NIH HHS R01 GM122951NIGMS NIH HHS R35 GM136433
6 · The paper itself

Abstract

During development, cells must quickly switch from one cell state to the next to execute precise and timely differentiation. One method to ensure fast transitions in cell states is by controlling gene expression at the post-transcriptional level through action of RNA-binding proteins on mRNAs. The ability to accurately identify the RNA targets of RNA-binding proteins at specific stages is key to understanding the functional role of RNA-binding proteins during development. Here we describe an adapted formaldehyde RNA immunoprecipitation (fRIP) protocol to identify the

Indexed as

fRIPImmunoprecipitationPost-transcriptional regulationProtein-RNA interactionsRNA-binding proteinsRNA targets

Identifiers

PMID35118177
PMCPMC8769761
OpenAlexW4205966708

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.