Evidence map›Paper›PMID 35118174›Full record

ArticleBio-protocol2022

A Simple Technique for Direct Immobilization of Target Enzymes from Cell Lysates Based on the SpyTag/SpyCatcher Spontaneous Reaction.

Li-Xi Cai, Yuan-Qing Lin, Yun-Meng Chu, Xiao-Pin Chen, Li-Xing Liu, Ming Zhang, Guang-Ya Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Li-Xi CaiCollege of Basic Medicine, Putian University, Putian, PR China.
Yuan-Qing LinDepartment of Bioengineering and Biotechnology, Huaqiao University, Xiamen, PR China.
Yun-Meng ChuDepartment of Bioengineering and Biotechnology, Huaqiao University, Xiamen, PR China.
Xiao-Pin ChenCollege of Basic Medicine, Putian University, Putian, PR China.
Li-Xing LiuCollege of Basic Medicine, Putian University, Putian, PR China.
Ming ZhangCollege of Basic Medicine, Putian University, Putian, PR China.
Guang-Ya ZhangDepartment of Bioengineering and Biotechnology, Huaqiao University, Xiamen, PR China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Many of the current methods for enzyme purification and immobilization suffer from several drawbacks, such as requiring tedious multistep procedures or long preparation, and being environmentally unfriendly, due to the chemicals and conditions involved. Thus, a simple technique for direct purification and immobilization of target enzymes from cell lysates was proposed. The elastin-like polypeptides (ELPs)-SpyCatcher chimera could mediate the formation of silica carriers within seconds and the target enzymes were then covalently immobilized on silica carriers via SpyCatcher/SpyTag spontaneous reaction. These tailor-made carriers were easily prepared, with precisely controlled morphology and size, as well as none-consuming surface modification needed, which could specifically immobilize the SpyTag-fused target enzymes from the cell lysate without pre-purification.

Indexed as

3-xylanaseElastin-like polypeptidesEnzyme immobilizationSilica nanoparticlesSpyCatcherSpyTagβ-1

Identifiers

PMID35118174
PMCPMC8769756

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.