ArticleTranslational cancer research2019
MicroRNA let-7a inhibits proliferation of breast cancer cell by downregulating USP32 expression.
Article in Translational cancer research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- The role of circulating MiR-34a-5p and MiR-let7a-5p as a diagnostic tumor markers in breast cancer a cross-sectional analysis.Molecular biology reports · 2026Article
- Identification of a Novel Salivary Four-miRNA Signature for Non-Invasive Diagnosis of Oral Squamous Cell Carcinoma.International journal of molecular sciences · 2025Article
- Phosphorylation of USP32 by CDK5 regulates Rap1 stability and therapeutic resistance in pancreatic ductal adenocarcinoma.Oncogene · 2025Article
- USP32 facilitates non-small cell lung cancer progression via deubiquitinating BAG3 and activating RAF-MEK-ERK signaling pathway.Oncogenesis · 2024Article
- Mapping the function of MicroRNAs as a critical regulator of tumor-immune cell communication in breast cancer and potential treatment strategies.Frontiers in cell and developmental biology · 2024Review
- USP32 deubiquitinase: cellular functions, regulatory mechanisms, and potential as a cancer therapy target.Cell death discovery · 2023Review
- Platelet-derived circRNAs signature in patients with gastroenteropancreatic neuroendocrine tumors.Journal of translational medicine · 2023Article
- Exploring the Potential Role of Circulating microRNAs as Biomarkers for Predicting Clinical Response to Neoadjuvant Therapy in Breast Cancer.International journal of molecular sciences · 2023Review
- Roles of the hsa_circ_0013880/USP32/Rap1b axis in the proliferation and apoptosis of acute myeloid leukemia cells.Acta biochimica et biophysica Sinica · 2023Article
- Photocaged amplified FRET nanoflares: spatiotemporal controllable of mRNA-powered nanomachines for precise and sensitive microRNA imaging in live cells.Nucleic acids research · 2022Article
- Downregulation of miR-182-5p inhibits the proliferation and invasion of triple-negative breast cancer cells through regulating TLR4/NF-κB pathway activity by targeting FBXW7.Annals of translational medicine · 2020Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe present study aimed to investigate the effect of microRNA (miR) let-7a on ubiquitin specific protease 32 (USP32) expression and its potential function in MCF-7 breast cancer (BCa) cell line.
methodsBCa MCF-7 cells were transfected with hsa-miR let-7a mimics or inhibitors, then the USP32 expression was evaluated using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and western blot analysis in the transfected cells. USP32 as a target regulated by miR let-7a was confirmed via Dual-luciferase reporter assay. The effects of miR let-7a on the viability were determined using MTT assay and colony formation analysis.
resultsWestern blot analysis revealed that miR let-7a mimics dramatically decreased the USP32 protein expression, whereas miR let-7a inhibitors increased the protein expression of USP32 compared with their controls in the MCF-7 cells. Dual-luciferase reporter assay showed that miR let-7a mimics could directly target the 3'-untranslated region (UTR) of USP32. Further, MTT assay and colony formation analysis showed that miR let-7a significantly inhibited cell proliferation of MCF-7 cells. However, overexpression of USP32 could reverse the effect of miR let-7a on MCF-7 cells proliferation.
conclusionsCollectively, the results suggested that miR let-7a functions as a tumor suppressor to reduce proliferation by targeting USP32 in BCa cells.
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