ArticleTranslational cancer research2021
The E3 protein ubiquitin ligase Itch is a potential target in myeloid malignancies with marrow fibrosis.
Article in Translational cancer research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed, 6 citations in OpenAlex.
- Proteome-wide identification and functional analysis of ubiquitinated proteins in Hepa1-6 cells by knockdown of E3 ubiquitin ligase SIAH1.Translational cancer research · 2024Article
- Crosstalk of ubiquitin system and non-coding RNA in fibrosis.International journal of biological sciences · 2024Review
- Role of macrophage polarization in osteoarthritis (Review).Experimental and therapeutic medicine · 2022Review
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Authors and funding
4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe underlying mechanism of myeloid malignancies like myelodysplastic syndrome (MDS) and acute myelocytic leukemia (AML) with bone marrow (BM) fibrosis (hereafter referred to as MDS-F and AML-F) is not fully understood. This study aimed to investigate the role of the E3 protein ubiquitin ligase Itch in the pathogenesis of these diseases preliminarily.
methodsThrough bioinformatic methods we found that the E3 protein ubiquitin ligase Itch might play a role in the pathogenesis of MDS-F and AML-F as well as the phosphatidylinositol 3-kinase (PI3K)/Akt pathway. We first investigated whether the PI3K/Akt pathway could regulate the expression Itch and its substrate p73 by using the myeloid neoplasm cell line K562 as a model. Then we assayed the Itch mRNA level of clinical samples in different subgroups to have a knowledge of its role in the myeloid diseases.
resultsThrough the cellular experiments we got that the PI3K/Akt pathway might not regulate the expression of Itch and its substrate p73. In patients with high risk MDS, AML, fibrosis or higher white blood cells (WBC) count, Itch mRNA level significantly increased when compared with the control groups. But the mRNA level didn't show significant difference in the subgroups classified by karyotype. Through correlative analysis we found that the mRNA level had positive correlation with the WBC count of the patients.
conclusionsThe PI3K/Akt pathway may not get involved in the regulation of the expression of Itch in K562 cells or myeloid tumors and Itch may play a role both in the proliferation and the generation of fibrosis in myeloid malignancies.
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