ArticleScientific reports2022
Detection of Mycobacterium tuberculosis complex DNA in oronasal swabs from infected African buffaloes (Syncerus caffer).
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Shedding-weighted network approaches for understanding tuberculosis maintenance in multihost systems using camera traps.Ecological applications : a publication of the Ecological Society of America · 2026Article
- Non-tuberculous mycobacteria in cattle: Distribution, antimicrobial resistance and zoonotic implications in Tamil Nadu, India.Veterinary research communications · 2026Article
- A microbial safari: finding evidence ofMicrobiology spectrum · 2026Article
- Culture-independent detection ofFrontiers in veterinary science · 2025Article
- Collaborative learning in the digital age: empowering tuberculosis researchers through virtual training.Journal of microbiology & biology education · 2024Article
- Identification and molecular characterization ofOne health (Amsterdam, Netherlands) · 2024Article
- Antemortem detection of Mycobacterium bovis in nasal swabs from African rhinoceros.Scientific reports · 2024Article
- Detection ofFrontiers in microbiology · 2024Article
- Diagnostic Evaluation of the IS1081-Targeted Real-Time PCR for Detection ofPathogens (Basel, Switzerland) · 2023Article
- Advancing animal tuberculosis surveillance using culture-independent long-read whole-genome sequencing.Frontiers in microbiology · 2023Article
- Identification and Characterisation of Nontuberculous Mycobacteria in African Buffaloes (Microorganisms · 2022Article
- Non-Coding RNAs in Tuberculosis Epidemiology: Platforms and Approaches for Investigating the Genome's Dark Matter.International journal of molecular sciences · 2022Review
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Authors and funding
4 authors.
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Abstract
Mycobacterium bovis (M. bovis), a member of the Mycobacterium tuberculosis complex (MTBC), is the causative agent of bovine TB (bTB) in animals. Spread occurs through inhalation or ingestion of bacilli transmitted from infected individuals. Early and accurate detection of infected African buffaloes shedding M. bovis is essential for interrupting transmission. In this pilot study, we determined if MTBC DNA could be detected in M. bovis infected buffalo oronasal secretions using a molecular transport media (PrimeStore MTM) with oronasal swabs and a rapid qPCR assay (Xpert MTB/RIF Ultra). Bovine TB test-positive buffaloes were culled, then tissue samples and oronasal swabs collected post-mortem for mycobacterial culture and Ultra testing, respectively. The Ultra detected MTBC DNA in 5/12 swabs from M. bovis culture-confirmed buffaloes. Oronasal swabs from M. bovis negative buffaloes (n = 20) were negative on Ultra, indicating the high specificity of this test. This study showed that MTM can successfully preserve MTBC DNA in oronasal swabs. The proportion of MTBC positive oronasal swabs was higher than expected and suggests that the Ultra may be an additional method for identifying infected buffaloes. Further studies are needed to confirm the utility of the Ultra assay with oronasal swabs as an assay to evaluate possible MTBC shedding in buffaloes.
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