ArticleBiological research2022
Neuronal GRK2 regulates microglial activation and contributes to electroacupuncture analgesia on inflammatory pain in mice.
Article in Biological research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Effects of combined transversus abdominis plane block under direct vision and acupoint injection on promoting rapid recovery after laparoscopic cholecystectomy.Langenbeck's archives of surgery · 2025Trial
- High numerical aperture confocal volumetric mesoscope reveals mesoscale subcellular dynamics in vivo.Nature biotechnology · 2026Article
- Regulation of G protein-coupled receptor kinase 2 and its role in tumors.Journal of translational medicine · 2026Review
- Stage-Specific Mechanisms of Manual Acupuncture and Electroacupuncture in Inflammatory Pain: A Time-Dependent Review.Journal of pain research · 2026Review
- Spinal dorsal horn IGF1 mediates the preventive effect of electroacupuncture on cisplatin-induced peripheral neuropathy via neuronal IGF1R in mice.Chinese medicine · 2025Article
- Electroacupuncture alleviates diabetic peripheral neuropathy through modulating mitochondrial biogenesis and suppressing oxidative stress.World journal of diabetes · 2025Article
- Genetic Evidence for Causal Effects of Immune Cell Subtypes on Postherpetic Neuralgia.Journal of pain research · 2025Article
- Microglia's Phenotypic Heterogeneity in Pain Pathogenesis and Electroacupuncture Analgesia: Mechanisms and Therapeutic Potential.Journal of pain research · 2025Review
- Recent advances in acupuncture for pain relief.Pain reports · 2024Article
- Electroacupuncture inhibited carrageenan-induced pain aversion by activating GABAergic neurons in the ACC.Molecular brain · 2024Article
- Acupuncture Effect on Reaction-Time Changes in Parkinson's Disease Patients-Case Study Series.Journal of clinical medicine · 2024Article
- Review
- Electroacupuncture attenuates neuropathic pain via suppressing BIP-IRE-1α-mediated endoplasmic reticulum stress in the anterior cingulate cortex.Biological research · 2024Article
- Andrographolide inhibits the activation of spinal microglia and ameliorates mechanical allodynia.Metabolic brain disease · 2024Article
- Hemoglobin α-derived peptides VD-hemopressin (α) and RVD-hemopressin (α) are involved in electroacupuncture inhibition of chronic pain.Frontiers in pharmacology · 2024Article
- Effects of Natural Product-Derived Compounds on Inflammatory Pain via Regulation of Microglial Activation.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Global Trends and Hotspots on Microglia Associated with Pain from 2002 to 2022: A Bibliometric Analysis.Journal of pain research · 2023Article
- Electroacupuncture alleviates orofacial allodynia and anxiety-like behaviors by regulating synaptic plasticity of the CA1 hippocampal region in a mouse model of trigeminal neuralgia.Frontiers in molecular neuroscience · 2022Article
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- Review
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundG protein coupled receptor kinase 2 (GRK2) has been demonstrated to play a crucial role in the development of chronic pain. Acupuncture is an alternative therapy widely used for pain management. In this study, we investigated the role of spinal neuronal GRK2 in electroacupuncture (EA) analgesia.
methodsThe mice model of inflammatory pain was built by subcutaneous injection of Complete Freund's Adjuvant (CFA) into the plantar surface of the hind paws. The mechanical allodynia of mice was examined by von Frey test. The mice were subjected to EA treatment (BL60 and ST36 acupuncture points) for 1 week. Overexpression and downregulation of spinal neuronal GRK2 were achieved by intraspinal injection of adeno associated virus (AAV) containing neuron-specific promoters, and microglial activation and neuroinflammation were evaluated by real-time PCR.
resultsIntraplantar injection with CFA in mice induced the decrease of GRK2 and microglial activation along with neuroinflammation in spinal cord. EA treatment increased the spinal GRK2, reduced neuroinflammation, and significantly decreased CFA-induced mechanical allodynia. The effects of EA were markedly weakened by non-cell-specific downregulation of spinal GRK2. Further, intraspinal injection of AAV containing neuron-specific promoters specifically downregulated neuronal GRK2, and weakened the regulatory effect of EA on CFA-induced mechanical allodynia and microglial activation. Meanwhile, overexpression of spinal neuronal GRK2 decreased mechanical allodynia. All these indicated that the neuronal GRK2 mediated microglial activation and neuroinflammation, and subsequently contributed to CFA-induced inflammatory pain.
conclusionThe restoration of the spinal GRK2 and subsequent suppression of microglial activation and neuroinflammation might be an important mechanism for EA analgesia. Our findings further suggested that the spinal GRK2, especially neuronal GRK2, might be the potential target for EA analgesia and pain management, and we provided a new experimental basis for the EA treatment of pain.
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