ArticlePlant biotechnology journal2022
Degradation of de-esterified pctin/homogalacturonan by the polygalacturonase GhNSP is necessary for pollen exine formation and male fertility in cotton.
Article in Plant biotechnology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 24 citations in OpenAlex.
- Long-term heat stress induces transcriptomic reprogramming of hormone signaling and metabolic pathways during rapeseed flowering.Scientific reports · 2025Article
- Key genes and molecular mechanisms responsible for male sterility revealed by transcriptome analysis in cotton.Plant cell reports · 2025Article
- GhAOS integrates circadian rhythm and jasmonic acid signaling to regulate high temperature stress responses in cotton and Arabidopsis.BMC biology · 2025Article
- Comprehensive study on softness mechanisms and the metabolic pattern of key cell wall components in tobacco leaves during the curing stage.BMC plant biology · 2025Article
- Genome-Wide Identification and Analysis of theGenes · 2024Article
- Single-Cell Transcriptome Atlas and Regulatory Dynamics in Developing Cotton Anthers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- A multi-organ maize metabolic model connects temperature stress with energy production and reducing power generation.iScience · 2023Article
- Histone H3 lysine 27 trimethylation suppresses jasmonate biosynthesis and signaling to affect male fertility under high temperature in cotton.Plant communications · 2023Article
- High-temperature stress in crops: male sterility, yield loss and potential remedy approaches.Plant biotechnology journal · 2023Review
- Cotton heterosis and hybrid cultivar development.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Integrated transcriptomic and proteomic analysis of a cytoplasmic male sterility line and associated maintainer line in soybean.Frontiers in plant science · 2023Article
- Degradation of de-esterified pctin/homogalacturonan by the polygalacturonase GhNSP is necessary for pollen exine formation and male fertility in cotton.Plant biotechnology journal · 2022Article
- Genomic and transcriptomic-based analysis of agronomic traits in sugar beet (Frontiers in plant science · 2022Article
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Authors and funding
18 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pollen wall exine provides a protective layer for the male gametophyte and is largely composed of sporopollenin, which comprises fatty acid derivatives and phenolics. However, the biochemical nature of the external exine is poorly understood. Here, we show that the male sterile line 1355A of cotton mutated in NO SPINE POLLEN (GhNSP) leads to defective exine formation. The GhNSP locus was identified through map-based cloning and confirmed by genetic analysis (co-segregation test and allele prediction using the CRISPR/Cas9 system). In situ hybridization showed that GhNSP is highly expressed in tapetum. GhNSP encodes a polygalacturonase protein homologous to AtQRT3, which suggests a function for polygalacturonase in pollen exine formation. These results indicate that GhNSP is functionally different from AtQRT3, the latter has the function of microspore separation. Biochemical analysis showed that the percentage of de-esterified pectin was significantly increased in the 1355A anthers at developmental stage 8. Furthermore, immunofluorescence studies using antibodies to the de-esterified and esterified homogalacturonan (JIM5 and JIM7) showed that the Ghnsp mutant exhibits abundant of de-esterified homogalacturonan in the tapetum and exine, coupled with defective exine formation. The characterization of GhNSP provides new understanding of the role of polygalacturonase and de-esterified homogalacturonan in pollen exine formation.
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