ArticleJournal of biomedical optics2022
First in patient assessment of brain tumor infiltrative margins using simultaneous time-resolved measurements of 5-ALA-induced PpIX fluorescence and tissue autofluorescence.
Article in Journal of biomedical optics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 22 citations in OpenAlex.
- NIR autofluorescence allows for pituitary gland detection during surgery: evidence from microscopic studies and in vivo measurements.Biomedical optics express · 2026Article
- Fluorescence lifetime imaging of 5-ALA-induced PpIX and autofluorescence for detecting infiltrating glioblastoma margins in patients.Biomedical optics express · 2026Article
- Label-free fluorescence lifetime imaging can distinguish cancer from healthy tissue in spontaneously occurring canine oral tumors.Scientific reports · 2026Article
- Imaging lymphatic function and inflammation response through hypoxia via endogenous biomarker.Journal of biomedical optics · 2026Article
- Light and metabolism: label-free optical imaging of metabolic activities in biological systems [Invited].Biomedical optics express · 2025Review
- Identification of colorectal malignancies enabled by phasor-based autofluorescence lifetime macroimaging and ensemble learning.Biophotonics discovery · 2025Article
- Towards Optical Biopsy in Glioma Surgery.International journal of molecular sciences · 2025Review
- Clinical confocal laser endomicroscopy for imaging of autofluorescence signals of human brain tumors and non-tumor brain.Journal of cancer research and clinical oncology · 2024Article
- Dual excitation spectral autofluorescence lifetime and reflectance imaging for fast macroscopic characterization of tissues.Biomedical optics express · 2024Article
- 5-ALA induced PpIX fluorescence spectroscopy in neurosurgery: a review.Frontiers in neuroscience · 2024Review
- Fluorescent Probes for Imaging in Humans: Where Are We Now?ACS nano · 2023Review
- Computational macroscopic lifetime imaging and concentration unmixing of autofluorescence.Journal of biophotonics · 2022Article
- 5-Aminolevulinic Acid-Induced Protoporphyrin IX Fluorescence Imaging for Tumor Detection: Recent Advances and Challenges.International journal of molecular sciences · 2022Review
- Fluorescence lifetime imaging of NAD(P)H upon oxidative stress inFrontiers in bioengineering and biotechnology · 2022Article
- Review
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
significance5-aminolevulinic acid (5-ALA)-induced protoporphyrin IX (PpIX) fluorescence is currently used for image-guided glioma resection. Typically, this widefield imaging method highlights the bulk of high-grade gliomas, but it underperforms at the infiltrating edge where PpIX fluorescence is not visible to the eyes. Fluorescence lifetime imaging (FLIm) has the potential to detect PpIX fluorescence below the visible detection threshold. Moreover, simultaneous acquisition of time-resolved nicotinamide adenine (phosphate) dinucleotide [NAD(P)H] fluorescence may provide metabolic information from the tumor environment to further improve overall tumor detection.
aimWe investigate the ability of pulse sampling, fiber-based FLIm to simultaneously image PpIX and NAD(P)H fluorescence of glioma infiltrative margins in patients. APPROACH: A mesoscopic fiber-based point-scanning FLIm device (355 nm pulses) was used to simultaneously resolve the fluorescence decay of PpIX (629/53 nm) and NAD(P)H (470/28 nm). The FLIm device enabled data acquisition at room light and rapid (<33 ms) augmentation of FLIm parameters on the surgical field-of-view. FLIm measurements from superficial tumors and tissue areas around the resection margins were performed on three glioblastoma patients in vivo following inspection of PpIX visible fluorescence with a conventional neurosurgical microscope. Microbiopsies were collected from FLIm imaged areas for histopathological evaluation.
resultsThe average lifetime from PpIX and NAD(P)H fluorescence distinguished between tumor and surrounding tissue. FLIm measurements of resection margins presented a range of PpIX and NAD(P)H lifetime values (τPpIX ∼ 3 to 14 ns, τNAD(P)H = 3 to 6 ns) associated with unaffected tissue and areas of low-density tumor infiltration.
conclusionsIntraoperative FLIm could simultaneously detect the emission of PpIX and NAD(P)H from patients in vivo during craniotomy procedures. This approach doubles as a clinical tool to identify tumor areas while performing tissue resection and as a research tool to study tumor microenvironmental changes in vivo. Intraoperative FLIm of 5-ALA-induced PpIX and tissue autofluorescence makes a promising surgical adjunct to guide tumor resection surgery.
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