Evidence map›Paper›PMID 35111902›Full record

ArticleACS central science2022

Molecular Prosthetics for Long-Term Functional Imaging with Fluorescent Reporters.

Vincent Grenier, Kayli N Martinez, Brittany R Benlian, Derek M García-Almedina, Benjamin K Raliski, Steven C Boggess, Johnathan C Maza, Samantha J Yang, Anneliese M M Gest, Evan W Miller

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS central science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Article
  2. Review
  3. A gentle palette of plasma membrane dyes.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  4. Review
  5. Article
  6. Article
  7. A red-emitting carborhodamine for monitoring and measuring membrane potential.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  8. Article
  9. Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Multi-color live-cell STED nanoscopy of mitochondria with a gentle inner membrane stain.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Vincent GrenierDepartment of Chemistry and Department of Molecular and Cell Biology and Helen Wills Neuroscience Institute. University of California, Berkeley, California 94720, United States.
Kayli N MartinezDepartment of Chemistry and Department of Molecular and Cell Biology and Helen Wills Neuroscience Institute. University of California, Berkeley, California 94720, United States.
Brittany R BenlianDepartment of Chemistry and Department of Molecular and Cell Biology and Helen Wills Neuroscience Institute. University of California, Berkeley, California 94720, United States.
Derek M García-AlmedinaDepartment of Chemistry and Department of Molecular and Cell Biology and Helen Wills Neuroscience Institute. University of California, Berkeley, California 94720, United States.ORCID https://orcid.org/0000-0002-0679-6930
Benjamin K RaliskiDepartment of Chemistry and Department of Molecular and Cell Biology and Helen Wills Neuroscience Institute. University of California, Berkeley, California 94720, United States.
Steven C BoggessDepartment of Chemistry and Department of Molecular and Cell Biology and Helen Wills Neuroscience Institute. University of California, Berkeley, California 94720, United States.
Johnathan C MazaDepartment of Chemistry and Department of Molecular and Cell Biology and Helen Wills Neuroscience Institute. University of California, Berkeley, California 94720, United States.ORCID https://orcid.org/0000-0003-2898-8770
Samantha J YangDepartment of Chemistry and Department of Molecular and Cell Biology and Helen Wills Neuroscience Institute. University of California, Berkeley, California 94720, United States.
Anneliese M M GestDepartment of Chemistry and Department of Molecular and Cell Biology and Helen Wills Neuroscience Institute. University of California, Berkeley, California 94720, United States.
Evan W MillerDepartment of Chemistry and Department of Molecular and Cell Biology and Helen Wills Neuroscience Institute. University of California, Berkeley, California 94720, United States.ORCID https://orcid.org/0000-0002-6556-7679

Funding

Univ of Calif Berkeley Chemistry-Biology Interface ProgramT32GM066698 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI CHANG, CHRISTOPHER J., FRANCIS, MATTHEW B · 2004 to 2023
$7.5M
New Chemical Tools for Exploring Cellular PhysiologyR35GM119855 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI MILLER, EVAN WALKER · 2016 to 2020
$1.3M
Upgrade for time-correlated single photon counting fluorescence lifetime laser scanning confocal microscopeS10OD025063 · OD · UNIVERSITY OF CALIFORNIA BERKELEY · PI AARON, HOLLY L · 2019 to 2019
$862k
NIGMS NIH HHS R35 GM119855NIGMS NIH HHS T32 GM066698NIH HHS S10 OD025063
6 · The paper itself

Abstract

Voltage-sensitive fluorescent reporters can reveal fast changes in the membrane potential in neurons and cardiomyocytes. However, in many cases, illumination in the presence of the fluorescent reporters results in disruptions to the action potential shape that limits the length of recording sessions. We show here that a molecular prosthetic approach, previously limited to fluorophores, rather than indicators, can be used to substantially prolong imaging in neurons and cardiomyocytes.

Identifiers

PMID35111902
PMCPMC8802189

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.