ReviewNature protocols2022
Fluorescent labeling of abundant reactive entities (FLARE) for cleared-tissue and super-resolution microscopy.
Review in Nature protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 22 citations in OpenAlex.
- Deep-learning triage of three-dimensional pathology datasets for comprehensive and efficient pathologist assessments.Nature biomedical engineering · 2026Article
- ONE microscopy.Nature protocols · 2026Review
- High-throughput, organ-scale 3D tubule tracking using TubuleMAP.Research square · 2026Article
- High-throughput, organ-scale 3D tubule tracking using TubuleMAP.bioRxiv : the preprint server for biology · 2026Article
- Expansion Microscopy reveals changes in cellular and extracellular structures between healthy and perturbed tendons.bioRxiv : the preprint server for biology · 2026Article
- Expansion and fluctuations-enhanced microscopy for nanoscale molecular profiling of cells and tissues.Nature protocols · 2026Review
- Super-resolved optical imaging, reconstruction, and spatial analysis of whole mouse glomeruli via the Glomerulus Mapping and Analysis Pipeline.Kidney international · 2025Article
- Highly accurate image registration for 3D multiplexed cyclic imaging using dense labeling in expandable tissue gels.PLoS biology · 2025Article
- Quantitative and comparative assessment of dyes and protocols for rapid ex vivo microscopy of fresh tissues.Scientific reports · 2024Article
- Single 5-nm quantum dot detection via microtoroid optical resonator photothermal microscopy.Light, science & applications · 2024Article
- Axially swept open-top light-sheet microscopy for densely labeled clinical specimens.Optics letters · 2024Article
- An end-to-end workflow for nondestructive 3D pathology.Nature protocols · 2024Review
- Differential labelling of human sub-cellular compartments with fluorescent dye esters and expansion microscopy.Nanoscale · 2023Article
- An end-to-end workflow for non-destructive 3D pathology.bioRxiv : the preprint server for biology · 2023Article
- Expansion microscopy: A chemical approach for super-resolution microscopy.Current opinion in structural biology · 2023Review
- Article
- Nondestructive 3D Pathology with Light-Sheet Fluorescence Microscopy for Translational Research and Clinical Assays.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Nanoscale fluorescence imaging of biological ultrastructure via molecular anchoring and physical expansion.Nano convergence · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Fluorescence microscopy is a vital tool in biomedical research but faces considerable challenges in achieving uniform or bright labeling. For instance, fluorescent proteins are limited to model organisms, and antibody conjugates can be inconsistent and difficult to use with thick specimens. To partly address these challenges, we developed a labeling protocol that can rapidly visualize many well-contrasted key features and landmarks on biological specimens in both thin and thick tissues or cultured cells. This approach uses established reactive fluorophores to label a variety of biological specimens for cleared-tissue microscopy or expansion super-resolution microscopy and is termed FLARE (fluorescent labeling of abundant reactive entities). These fluorophores target chemical groups and reveal their distribution on the specimens; amine-reactive fluorophores such as hydroxysuccinimidyl esters target accessible amines on proteins, while hydrazide fluorophores target oxidized carbohydrates. The resulting stains provide signals analogous to traditional general histology stains such as H&E or periodic acid-Schiff but use fluorescent probes that are compatible with volumetric imaging. In general, the stains for FLARE are performed in the order of carbohydrates, amine and DNA, and the incubation time for the stains varies from 1 h to 1 d depending on the combination of stains and the type and thickness of the biological specimens. FLARE is powerful, robust and easy to implement in laboratories that already routinely do fluorescence microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.