ArticleScientific reports2022
Advanced sequencing approaches detected insertions of viral and human origin in the viral genome of chronic hepatitis E virus patients.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 17 citations in OpenAlex.
- Impact of hepatitis E virus host- and virus-derived insertions on the antiviral response in primary human hepatocytes.bioRxiv : the preprint server for biology · 2026Article
- Identification of novel rodent and shrew orthohepeviruses sheds light on hepatitis E virus evolution.Zoological research · 2025Article
- Comparative Evaluation of Open-Source Bioinformatics Pipelines for Full-Length Viral Genome Assembly.Viruses · 2024Article
- Article
- Article
- Characterization of virus‒host recombinant variants of the hepatitis E virus.Journal of virology · 2024Article
- Whole genome sequencing reveals insights into hepatitis E virus genome diversity, and virus compartmentalization in chronic hepatitis E.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2023Article
- Review
- Risk Factors for Hepatitis E Virus Infection and Eating Habits in Kidney Transplant Recipients.Pathogens (Basel, Switzerland) · 2023Article
- Expanding the Hepatitis E Virus Toolbox: Selectable Replicons and Recombinant Reporter Genomes.Viruses · 2023Review
- In Vitro Replication of Swine Hepatitis E Virus (HEV): Production of Cell-Adapted Strains.Animals : an open access journal from MDPI · 2023Article
- Genetic Evolution of Hepatitis E Virus.Advances in experimental medicine and biology · 2023Review
- Micro RNAs-The Small Big Players in Hepatitis E Virus Infection: A Comprehensive Review.Biomolecules · 2022Review
- Article
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Authors and funding
13 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The awareness of hepatitis E virus (HEV) increased significantly in the last decade due to its unexpectedly high prevalence in high-income countries. There, infections with HEV-genotype 3 (HEV-3) are predominant which can progress to chronicity in immunocompromised individuals. Persistent infection and antiviral therapy can select HEV-3 variants; however, the spectrum and occurrence of HEV-3 variants is underreported. To gain in-depth insights into the viral population and to perform detailed characterization of viral genomes, we used a new approach combining long-range PCR with next-generation and third-generation sequencing which allowed near full-length sequencing of HEV-3 genomes. Furthermore, we developed a targeted ultra-deep sequencing approach to assess the dynamics of clinically relevant mutations in the RdRp-region and to detect insertions in the HVR-domain in the HEV genomes. Using this new approach, we not only identified several insertions of human (AHNAK, RPL18) and viral origin (RdRp-derived) in the HVR-region isolated from an exemplary sample but detected a variant containing two different insertions simultaneously (AHNAK- and RdRp-derived). This finding is the first HEV-variant recognized as such showing various insertions in the HVR-domain. Thus, this molecular approach will add incrementally to our current knowledge of the HEV-genome organization and pathogenesis in chronic hepatitis E.
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