ArticleCurrent biology : CB2022
Non-centrosomal microtubules at kinetochores promote rapid chromosome biorientation during mitosis in human cells.
Article in Current biology : CB, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 53 citations in OpenAlex.
- Structure and function of the outer kinetochore.Nature reviews. Molecular cell biology · 2026Review
- Microtubule depolymerization at kinetochores restricts anaphase spindle elongation.Nature chemical biology · 2026Article
- Article
- Polar chromosomes are rescued from missegregation by spindle elongation-driven microtubule pivoting.Nature communications · 2026Article
- The kinetochore corona orchestrates chromosome congression through transient microtubule interactions.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Tau condensation on DNA mediates microtubule attachment suggesting a mitotic role for centromere-localized tau.Nature communications · 2026Article
- Kinetochore-centrosome feedback linking CENP-E and Aurora kinases controls chromosome congression.Nature communications · 2025Article
- CENP-E initiates chromosome congression by opposing Aurora kinases to promote end-on attachments.Nature communications · 2025Article
- Branching, crosslinking, and decentralization of microtubules accelerates intracellular assembly.Biophysical journal · 2025Article
- Proximity-based activation of AURORA A by MPS1 potentiates error correction.Current biology : CB · 2025Article
- Acentric chromosome congression and alignment on the metaphase plate via kinetochore-independent forces.Genetics · 2025Article
- Ipsilateral restriction of chromosome movement along a centrosome, and apical-basal axis during the cell cycle.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Article
- An Arabidopsis Kinesin-14D motor is associated with midzone microtubules for spindle morphogenesis.Current biology : CB · 2024Article
- Meiosis-specific functions of kinetochore protein SPC105R required for chromosome segregation inMolecular biology of the cell · 2024Article
- Measuring and modeling the dynamics of mitotic error correction.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Antiparallel microtubule bundling supports KIF15-driven mitotic spindle assembly.Molecular biology of the cell · 2024Article
- Ipsilateral restriction of chromosome movement along a centrosome, and apical-basal axis during the cell cycle.Research square · 2024Article
- CKAP5 stabilizes CENP-E at kinetochores by regulating microtubule-chromosome attachments.EMBO reports · 2024Article
- Meiosis-specific functions of kinetochore protein SPC105R required for chromosome segregation inbioRxiv : the preprint server for biology · 2024Article
- Microtubule nucleation for spindle assembly: one molecule at a time.Trends in biochemical sciences · 2023Review
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8 authors at 4 institutions in 1 country.
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Abstract
Proper segregation of chromosomes during mitosis depends on "amphitelic attachments"-load-bearing connections of sister kinetochores to the opposite spindle poles via bundles of microtubules, termed as the "K-fibers." Current models of spindle assembly assume that K-fibers arise largely from stochastic capture of microtubules, which occurs at random times and locations and independently at sister kinetochores. We test this assumption by following the movements of all kinetochores in human cells and determine that most amphitelic attachments form synchronously at a specific stage of spindle assembly and within a spatially distinct domain. This biorientation domain is enriched in bundles of antiparallel microtubules, and perturbation of microtubule bundling changes the temporal and spatial dynamics of amphitelic attachment formation. Structural analyses indicate that interactions of kinetochores with microtubule bundles are mediated by non-centrosomal short microtubules that emanate from most kinetochores during early prometaphase. Computational analyses suggest that momentous molecular motor-driven interactions with antiparallel bundles rapidly convert these short microtubules into nascent K-fibers. Thus, load-bearing connections to the opposite spindle poles form simultaneously on sister kinetochores. In contrast to the uncoordinated sequential attachments of sister kinetochores expected in stochastic models of spindle assembly, our model envisions the formation of amphitelic attachments as a deterministic process in which the chromosomes connect with the spindle poles synchronously at a specific stage of spindle assembly and at a defined location determined by the spindle architecture. Experimental analyses of changes in the kinetochore behavior in cells with perturbed activity of molecular motors CenpE and dynein confirm the predictive power of the model.
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