ArticleeLife2022
Rolling circle RNA synthesis catalyzed by RNA.
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Molecular insights into dynamic RNA quaternary assemblies.RNA biology · 2026Review
- Exonuclease Ribozyme Encoded in a Circular Genome: On the Initiation of the RNA World.Life (Basel, Switzerland) · 2026Article
- Trinucleotide substrates under pH-freeze-thaw cycles enable open-ended exponential RNA replication by a polymerase ribozyme.Nature chemistry · 2025Article
- Roles of dimeric intermediates in RNA-catalyzed rolling circle synthesis.Nucleic acids research · 2025Article
- Origin of the RNA World in Cold Hadean Geothermal Fields Enriched in Zinc and Potassium: Abiogenesis as a Positive Fallout from the Moon-Forming Impact?Life (Basel, Switzerland) · 2025Article
- Perspective: Protocells and the Path to Minimal Life.Journal of molecular evolution · 2024Review
- Architecture of an RNA polymerase ribozyme illuminates the RNA World.Trends in genetics : TIG · 2024Article
- Cryo-EM structure and functional landscape of an RNA polymerase ribozyme.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Circular at the very beginning: on the initial genomes in the RNA world.RNA biology · 2024Article
- Experimental Tests of the Virtual Circular Genome Model for Nonenzymatic RNA Replication.Journal of the American Chemical Society · 2023Article
- Ribozyme-mediated RNA synthesis and replication in a model Hadean microenvironment.Nature communications · 2023Article
- Towards an RNA/Peptides World by the Direct RNA Template Mechanism: The Emergence of Membrane-Stabilizing Peptides in RNA-Based Protocells.Life (Basel, Switzerland) · 2023Article
- Primitive Oligomeric RNAs at the Origins of Life on Earth.International journal of molecular sciences · 2023Article
- The Ring World: Eversion of Small Double-Stranded Polynucleotide Circlets at the Origin of DNA Double Helix, RNA Polymerization, Triplet Code, Twenty Amino Acids, and Strand Asymmetry.International journal of molecular sciences · 2022Article
- Rolling Circles as a Means of Encoding Genes in the RNA World.Life (Basel, Switzerland) · 2022Article
- The Effect of Environment on the Evolution and Proliferation of Protocells of Increasing Complexity.Life (Basel, Switzerland) · 2022Article
- Computer simulations of Template-Directed RNA Synthesis driven by temperature cycling in diverse sequence mixtures.PLoS computational biology · 2022Article
- Visualizing RNA polymers produced by hot wet-dry cycling.Scientific reports · 2022Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
RNA-catalyzed RNA replication is widely considered a key step in the emergence of life's first genetic system. However, RNA replication can be impeded by the extraordinary stability of duplex RNA products, which must be dissociated for re-initiation of the next replication cycle. Here, we have explored rolling circle synthesis (RCS) as a potential solution to this strand separation problem. We observe sustained RCS by a triplet polymerase ribozyme beyond full-length circle synthesis with strand displacement yielding concatemeric RNA products. Furthermore, we show RCS of a circular Hammerhead ribozyme capable of self-cleavage and re-circularization. Thus, all steps of a viroid-like RNA replication pathway can be catalyzed by RNA alone. Finally, we explore potential RCS mechanisms by molecular dynamics simulations, which indicate a progressive build-up of conformational strain upon RCS with destabilization of nascent strand 5'- and 3'-ends. Our results have implications for the emergence of RNA replication and for understanding the potential of RNA to support complex genetic processes.
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