ArticleAnalytical chemistry2022
Optimized Sawhorse Waveform for the Measurement of Oxytocin Release in Zebrafish.
Article in Analytical chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 10 citations in OpenAlex.
- Quantitative Control of ZnAnalytical chemistry · 2025Article
- Voltammetric detection of Neuropeptide Y using a modified sawhorse waveform.Analytical and bioanalytical chemistry · 2024Article
- Perspective-Advances in Voltammetric Methods for the Measurement of Biomolecules.ECS sensors plus · 2024Article
- In Vitro Biofouling Performance of Boron-Doped Diamond Microelectrodes for Serotonin Detection Using Fast-Scan Cyclic Voltammetry.Biosensors · 2023Article
- Dopamine Release Impairments Accompany Locomotor and Cognitive Deficiencies in Rotenone-Treated Parkinson's Disease Model Zebrafish.Chemical research in toxicology · 2022Article
- Impaired Dopamine Release and Latent Learning in Alzheimer's Disease Model Zebrafish.ACS chemical neuroscience · 2022Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Oxytocin is a nonapeptide hormone involved in numerous physiological functions. Real-time electrochemical measurements of oxytocin in living tissue are challenging due to electrode fouling and the large potentials needed to oxidize the tyrosine residue. Here, we used fast-scan cyclic voltammetry at carbon-fiber microelectrodes and flow injection analysis to optimize a waveform for the measurement of oxytocin. This optimized waveform employed an accumulation potential of -0.6 V, multiple scan rates, and a 3 ms holding potential at a positive, oxidizing potential of +1.4 V before linearly scanning the potential back to -0.6 V (versus Ag/AgCl). We obtained a limit of quantitation of 0.34 ± 0.02 μM, and our electrodes did not foul upon multiple injections. Moreover, to demonstrate the utility of our method, we measured the release of oxytocin, evoked by light application and mechanical perturbation, in whole brains from genetically engineered adult zebrafish that express channelrhodopsin-2 selectively on oxytocinergic neurons. Collectively, this work expands the toolkit for the measurement of peptides in living tissue preparations.
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Registered trials
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