ArticleClinical and experimental dental research2022
The profiling and analysis of gene expression in human periodontal ligament tissue and fibroblasts.
Article in Clinical and experimental dental research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- In Vitro Evaluation of the Regenerative Potential of Autologous Platelet-Rich Fibrin (PRF) on Human Primary Periodontal Ligament Cells.International journal of molecular sciences · 2025Article
- Impact of Atomic Layer-Deposited Hydroxyapatite-Coated Titanium on Expression of Focal Adhesion Molecules of Human Gingival Fibroblasts.Nanomaterials (Basel, Switzerland) · 2025Article
- Formulation of Hyperelastic Constitutive Model for Human Periodontal Ligament Based on Fiber Volume Fraction.Materials (Basel, Switzerland) · 2025Article
- A bioreactor-based platform for investigating the early response of human periodontal ligament stem cells to intermittent mechanical stretching.Frontiers in bioengineering and biotechnology · 2025Article
- Article
- The construction of a microenvironment with the vascular network by co-culturing fibroblasts and endothelial cells.Regenerative therapy · 2024Article
- Control of tissue homeostasis by the extracellular matrix: Synthetic heparan sulfate as a promising therapeutic for periodontal health and bone regeneration.Periodontology 2000 · 2024Review
- Multiomics analysis of cultured mouse periodontal ligament cell-derived extracellular matrix.Scientific reports · 2024Article
- Decorin Promotes Osteoblastic Differentiation of Human Periodontal Ligament Stem Cells.Molecules (Basel, Switzerland) · 2022Article
- Novel In Situ-Cross-Linked Electrospun Gelatin/Hydroxyapatite Nonwoven Scaffolds Prove Suitable for Periodontal Tissue Engineering.Pharmaceutics · 2022Article
- The profiling and analysis of gene expression in human periodontal ligament tissue and fibroblasts.Clinical and experimental dental research · 2022Article
- The profiling and analysis of gene expression in rat temporomandibular joint disc tissue and its derived cells.Journal of oral biology and craniofacial researchArticle
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesThe periodontal ligament (PDL) is an important component of periodontium to support dental structure in the alveolar socket. Regeneration of PDL tissue is an effective treatment option for periodontal disease and the profiling of genes involved in this process will be informative. Therefore, our study aims to accurately delineate the profiling of gene expression for PDL tissue regeneration. MATERIALS AND
methodsWe isolated PDL tissues and PDL fibroblasts (PDLFs) from premolar teeth, which were extracted from healthy periodontal status patients undergoing orthodontic treatment. Messenger RNA (mRNA) expression in PDL tissue and PDLFs were analyzed using Cap analysis gene expression, which is a second-generation sequencing technique to create profiling. We also determined the protein expression using Western blot.
resultsCollagens (type I, III, and VI), noncollagenous proteins (periostin and osteonectin), and proteoglycans (asporin, lumican, decorin, and osteomodulin) were highly expressed in PDL tissue. Integrin, β1 was also expressed in PDL tissue. On comparison of gene expression between PDL tissue and PDLFs, four PDL marker genes, osteopontin, asporin, periostin, and osteonectin, were decreased in PDLFs. The genes for gene regulation were also highly expressed.
conclusionsOur study demonstrated the overall profiling of mRNA expression in PDL tissue and analyzed the important genes which may be useful for providing specific information for the reconstruction of PDL. We also identified the difference in gene expression between PDL tissue and PDLFs which might provide insights towards PDL regeneration.
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