ArticleFolia microbiologica2022
A highly efficient stratagem for protoplast isolation and genetic transformation in filamentous fungus Colletotrichum falcatum.
Article in Folia microbiologica, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 15 citations in OpenAlex.
- Establishment of the Genetic Transformation System ofJournal of fungi (Basel, Switzerland) · 2026Article
- An Efficient and Stable PEG-Mediated Transformation System for Medicinal FungusJournal of fungi (Basel, Switzerland) · 2026Article
- PEG-mediated transformation: a tool for random integration or targeted gene replacement inFrontiers in microbiology · 2026Article
- Fungal Coculture: Unlocking the Potential for Efficient Bioconversion of Lignocellulosic Biomass.Journal of fungi (Basel, Switzerland) · 2025Review
- PEG-Mediated Protoplast Transformation ofJournal of fungi (Basel, Switzerland) · 2025Article
- Extracting Protoplasts from Filamentous Fungi Using Extralyse, An Enzyme Used in the Wine Industry.Current protocols · 2025Article
- Comparative Genomic Analysis of Two Monokaryons ofJournal of fungi (Basel, Switzerland) · 2025Article
- Tracking the infection dynamics ofFrontiers in plant science · 2025Article
- The Mechanism of Transcription Factor Swi6 in Regulating Growth and Pathogenicity ofMicroorganisms · 2023Article
- Development ofFrontiers in microbiology · 2022Article
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Authors and funding
8 authors at 2 institutions in 1 country.
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Abstract
Red rot of sugarcane caused by the hemi-biotrophic fungal pathogen, Colletotrichum falcatum, is a major threat to sugarcane cultivation in many tropical countries such as India, Bangladesh, and Pakistan. With the accumulating information on pathogenicity determinants, namely, effectors and pathogen-associated molecular patterns (PAMPs) of C. falcatum, it is of paramount importance to decipher the functional role of these molecular players that may ultimately decide upon the outcome of sugarcane-C. falcatum interaction. Since C. falcatum is a multinucleated filamentous fungus, the conventional Agrobacterium-mediated transformation method could not be effectively utilized for targeted manipulation of genomic DNA. Hence, we developed a highly efficient protoplast-based transformation method for the virulent pathotype of C. falcatum - Cf671, which involves isolation of protoplast, polyethylene glycol (PEG)-mediated transformation, and regeneration of transformed protoplasts into hyphal colonies. In this study, germinating conidiospores of Cf671 were treated with different enzyme-osmoticum combinations, out of which 20 mg/mL lysing enzyme with 5 mg/mL β-glucanase in an osmoticum of 1.2 mol/L MgSO
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