Evidence map›Paper›PMID 35106614›Full record

ArticleAnalytical and bioanalytical chemistry2022

Rapid detection and quantitation of dipicolinic acid from Clostridium botulinum spores using mixed-mode liquid chromatography-tandem mass spectrometry.

Benjamin W Redan, Travis R Morrissey, Catherine A Rolfe, Viviana L Aguilar, Guy E Skinner, N Rukma Reddy

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.4field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 20 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Benjamin W RedanDivision of Food Processing Science and Technology, Office of Food Safety, U.S. Food and Drug Administration, 6502 South Archer Road, Bedford Park, IL, USA. benjamin.redan@fda.hhs.gov.ORCID http://orcid.org/0000-0002-2834-4468
Travis R MorrisseyDivision of Food Processing Science and Technology, Office of Food Safety, U.S. Food and Drug Administration, 6502 South Archer Road, Bedford Park, IL, USA.
Catherine A RolfeDivision of Food Processing Science and Technology, Office of Food Safety, U.S. Food and Drug Administration, 6502 South Archer Road, Bedford Park, IL, USA.
Viviana L AguilarInstitute for Food Safety and Health, Illinois Institute of Technology, 6502 South Archer Road, Bedford Park, IL, USA.
Guy E SkinnerMulti-Component Foods, Food Process Evaluation Team, Office of Food Safety, U.S. Food and Drug Administration, 5001 Campus Drive, College Park, MD, USA.
N Rukma ReddyDivision of Food Processing Science and Technology, Office of Food Safety, U.S. Food and Drug Administration, 6502 South Archer Road, Bedford Park, IL, USA.
United States Food and Drug Administration · USCenter for Food Safety and Applied Nutrition · USIllinois Institute of Technology · US

Funding

FDA HHS U19 FD005322Intramural FDA HHS FD999999
6 · The paper itself

Abstract

Analysis of the dipicolinic acid (DPA) released from Clostridium botulinum spores during thermal processing is crucial to obtaining a mechanistic understanding of the factors involved in spore heat resistance and related food safety applications. Here, we developed a novel mixed-mode liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for detection of the DPA released from C. botulinum type A, nonproteolytic types B and F strains, and nonpathogenic surrogate Clostridium sporogenes PA3679 spores. DPA was retained on a mixed-mode C18/anion exchange column and was detected using electrospray ionization (ESI) positive mode within a 4-min analysis time. The intraday and interday precision (%CV) was 1.94-3.46% and 4.04-8.28%, respectively. Matrix effects were minimal across proteolytic type A Giorgio-A, nonproteolytic types QC-B and 202-F, and C. sporogenes PA3679 spore suspensions (90.1-114% of spiked DPA concentrations). DPA recovery in carrot juice and beef broth ranged from 105 to 118%, indicating limited matrix effects of these food products. Experiments that assessed the DPA released from Giorgio-A spores over the course of a 5-min thermal treatment at 108 °C found a significant correlation (R = 0.907; P < 0.05) between the log reduction of spores and amount of DPA released. This mixed-mode LC-MS/MS method provides a means for rapid detection of DPA released from C. botulinum spores during thermal processing and has the potential to be used for experiments in the field of food safety that assess the thermal resistance characteristics of various C. botulinum spore types.

Indexed as

Clostridium botulinumPicolinic AcidsChromatography, LiquidHot TemperatureSpores, BacterialTandem Mass Spectrometrydipicolinic acidPicolinic AcidsBotulismEndosporesFood safetyLC–MS/MSPyridine-2,6-dicarboxylic acidThermal processing

Identifiers

PMID35106614
PMCPMC9109463
OpenAlexW4210253627

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.