Evidence map›Paper›PMID 35106155›Full record

ArticleClinical & translational immunology2022

DROSHA but not DICER is required for human haematopoietic stem cell function.

Karen Gu, Carina Walpole, Shayarana Gooneratne, Xin Liu, Oscar L Haigh, Kristen J Radford, Mark Mw Chong

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical & translational immunology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Karen GuSt Vincent's Institute of Medical Research Fitzroy VIC 3065 Australia.
Carina WalpoleMater Research Institute Translational Research Institute The University of Queensland Woolloongabba QLD 4102 Australia.
Shayarana GooneratneSt Vincent's Institute of Medical Research Fitzroy VIC 3065 Australia.
Xin LiuSt Vincent's Institute of Medical Research Fitzroy VIC 3065 Australia.
Oscar L HaighMater Research Institute Translational Research Institute The University of Queensland Woolloongabba QLD 4102 Australia.
Kristen J RadfordMater Research Institute Translational Research Institute The University of Queensland Woolloongabba QLD 4102 Australia.ORCID https://orcid.org/0000-0001-6512-6323
Mark Mw ChongSt Vincent's Institute of Medical Research Fitzroy VIC 3065 Australia.ORCID https://orcid.org/0000-0002-3701-7397
Translational Research Institute · AUSt Vincents Institute of Medical Research · AUThe University of Melbourne · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesDROSHA and DICER have central roles in the biogenesis of microRNAs (miRNAs). However, we previously showed that in the murine system, DROSHA has an alternate function where it directly recognises and cleaves protein-coding messenger (m)RNAs and this is critical for safeguarding the pluripotency of haematopoietic stem cells (HSCs). Maintenance of murine HSC function is dependent on DROSHA-mediated cleavage of two mRNAs,

methodsDROSHA and DICER were knocked down in human cord blood CD34

resultsConsistent with murine HSCs, DROSHA knockdown impaired the differentiation of human HSCs

conclusionA miRNA-independent function of DROSHA is critical for the function of human HSCs. DROSHA directly recognises and degrades mRNAs in humans HSCs. However, unlike in murine HSCs, the degradation of the MYL9 mRNA alone is not critical for human HSC function. Therefore, DROSHA must be inhibiting other targets and/or has another miRNA-independent function that is essential for safeguarding the pluripotency of human HSCs.

Indexed as

DICERDROSHAhaematopoietic stem cellsmicroRNAmRNA

Identifiers

PMID35106155
PMCPMC8784585
OpenAlexW4207010828

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.