ArticleThe ISME journal2022
Cultivation and biogeochemical analyses reveal insights into methanogenesis in deep subseafloor sediment at a biogenic gas hydrate site.
Article in The ISME journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 42 citations in OpenAlex.
- Methane dynamics in gas chimneys linking geochemical and microbial methane cycling in the Ulleung basin.BMC microbiology · 2025Article
- The Meso- and Bathypelagic Archaeal and Bacterial Communities of the Southern Gulf of Mexico Are Dominated by Nitrifiers and Hydrocarbon Degraders.Microorganisms · 2025Article
- High-pressure Methanogenesis Reveals Metabolic Adaptation to Dissolved COMicrobes and environments · 2025Article
- Article
- Hydrochemical gradients driving extremophile distribution in saline and brine groundwater of southern Poland.Environmental microbiology reports · 2024Article
- Potential coupling of microbial methane, nitrogen, and sulphur cycling in the Okinawa Trough cold seep sediments.Microbiology spectrum · 2024Article
- Well-hidden methanogenesis in deep, organic-rich sediments of Guaymas Basin.The ISME journal · 2023Article
- Vertically stratified methane, nitrogen and sulphur cycling and coupling mechanisms in mangrove sediment microbiomes.Microbiome · 2023Article
- Revisiting Microbial Diversity in Hypersaline Microbial Mats from Guerrero Negro for a Better Understanding of Methanogenic Archaeal Communities.Microorganisms · 2023Article
- The majority of microorganisms in gas hydrate-bearing subseafloor sediments ferment macromolecules.Microbiome · 2023Article
- Phenotypic and genomic characterization of the first alkaliphilic aceticlastic methanogens and proposal of a novel genusFrontiers in microbiology · 2023Article
- Methanogen activity and microbial diversity in Gulf of Cádiz mud volcano sediments.Frontiers in microbiology · 2023Article
- Microbial communities associated with thermogenic gas hydrate-bearing marine sediments in Qiongdongnan Basin, South China Sea.Frontiers in microbiology · 2022Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gas hydrates deposited in subseafloor sediments are considered to primarily consist of biogenic methane. However, little evidence for the occurrence of living methanogens in subseafloor sediments has been provided. This study investigated viable methanogen diversity, population, physiology and potential activity in hydrate-bearing sediments (1-307 m below the seafloor) from the eastern Nankai Trough. Radiotracer experiments, the quantification of coenzyme F430 and molecular sequencing analysis indicated the occurrence of potential methanogenic activity and living methanogens in the sediments and the predominance of hydrogenotrophic methanogens followed by methylotrophic methanogens. Ten isolates and nine representative culture clones of hydrogenotrophic, methylotrophic and acetoclastic methanogens were obtained from the batch incubation of sediments and accounted for 0.5-76% of the total methanogenic sequences directly recovered from each sediment. The hydrogenotrophic methanogen isolates of Methanocalculus and Methanoculleus that dominated the sediment methanogen communities produced methane at temperatures from 4 to 55 °C, with an abrupt decline in the methane production rate at temperatures above 40 °C, which is consistent with the depth profiles of potential methanogenic activity in the Nankai Trough sediments in this and previous studies. Our results reveal the previously overlooked phylogenetic and metabolic diversity of living methanogens, including methylotrophic methanogenesis.
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