ArticleNature communications2022
SARS-CoV-2 genomes from Saudi Arabia implicate nucleocapsid mutations in host response and increased viral load.
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Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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45 citing papers in PubMed, 63 citations in OpenAlex.
- SARS-CoV-2 nucleocapsid protein hijacks host transcription by targeting eEF1A1.Life science alliance · 2026Article
- Combinatorial analysis of clinical and genomic data used to assess the association between SARS-CoV-2 mutations and disease severity.PNAS nexus · 2026Article
- Striatal transcriptomic alterations immediately after short-term abstinence from methamphetamine self-administration in rats.Molecular brain · 2025Article
- Evolution of Antiviral Drug Resistance in SARS-CoV-2.Viruses · 2025Review
- Coupling of SARS-CoV-2 to Amyloid Fibrils and Liquid-Liquid Phase Separation.Current protein & peptide science · 2025Review
- The R203M and D377Y mutations of the nucleocapsid protein promote SARS-CoV-2 infectivity by impairing RIG-I-mediated antiviral signaling.PLoS pathogens · 2025Article
- Strategies Used by SARS-CoV-2 to Evade the Innate Immune System in an Evolutionary Perspective.Pathogens (Basel, Switzerland) · 2024Review
- A core network in the SARS-CoV-2 nucleocapsid NTD mediates structural integrity and selective RNA-binding.Nature communications · 2024Article
- Comparative Evolutionary Epidemiology of SARS-CoV-2 Delta and Omicron Variants in Kuwait.Viruses · 2024Article
- Modulation of UPF1 catalytic activity upon interaction of SARS-CoV-2 Nucleocapsid protein with factors involved in nonsense mediated-mRNA decay.Nucleic acids research · 2024Article
- SARS-CoV-2 N protein coordinates viral particle assembly through multiple domains.Journal of virology · 2024Article
- Early detection of highly transmissible viral variants using phylogenomics.Science advances · 2024Article
- Review
- Impact of mutations on the stability of SARS-CoV-2 nucleocapsid protein structure.Scientific reports · 2024Article
- Genomic Surveillance and Mutation Analysis of SARS-CoV-2 Variants among Patients in Saudi Arabia.Microorganisms · 2024Article
- Integration of individualized and population-level molecular epidemiology data to model COVID-19 outcomes.Cell reports. Medicine · 2024Article
- Differential Type-I Interferon Response in Buffy Coat Transcriptome of Individuals Infected with SARS-CoV-2 Gamma and Delta Variants.International journal of molecular sciences · 2023Article
- Phylogenies increase power to detect highly transmissible viral genome variants.medRxiv : the preprint server for health sciences · 2023Article
- Impact of the SARS-CoV-2 nucleocapsid 203K/204R mutations on the inflammatory immune response in COVID-19 severity.Genome medicine · 2023Article
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Authors and funding
36 authors at 11 institutions in 3 countries.
Tobias Mourier *King Abdullah University of Science and Technology (KAUST), Pathogen Genomics Laboratory, Biological and Environmental Science and Engineering (BESE), Thuwal-Jeddah, 23955-6900, Saudi Arabia.ORCID 0000-0003-2727-1903
Muhammad Shuaib *King Abdullah University of Science and Technology (KAUST), Pathogen Genomics Laboratory, Biological and Environmental Science and Engineering (BESE), Thuwal-Jeddah, 23955-6900, Saudi Arabia.ORCID 0000-0001-6238-7448
Sharif Hala *Infectious Disease Research Department, King Abdullah International Medical Research Centre, Ministry of National Guard Health Affairs, Jeddah, Saudi Arabia.
Sara Mfarrej *King Abdullah University of Science and Technology (KAUST), Pathogen Genomics Laboratory, Biological and Environmental Science and Engineering (BESE), Thuwal-Jeddah, 23955-6900, Saudi Arabia.ORCID 0000-0002-6460-8862
Fadwa AlofiInfectious Diseases Department, King Fahad Hospital, Madinah, MOH, Saudi Arabia.
Raeece NaeemKing Abdullah University of Science and Technology (KAUST), Pathogen Genomics Laboratory, Biological and Environmental Science and Engineering (BESE), Thuwal-Jeddah, 23955-6900, Saudi Arabia.ORCID 0000-0003-1734-1007
Afrah AlsomaliInfectious Diseases Department, King Abdullah Medical Complex, Jeddah, MOH, Saudi Arabia.
David JorgensenSchool of Public Health, Faculty of Medicine, Imperial College, Norfolk Place, St Mary's Campus, London, United Kingdom.
Amit Kumar SubudhiKing Abdullah University of Science and Technology (KAUST), Pathogen Genomics Laboratory, Biological and Environmental Science and Engineering (BESE), Thuwal-Jeddah, 23955-6900, Saudi Arabia.ORCID 0000-0002-3079-3705
Fathia Ben RachedKing Abdullah University of Science and Technology (KAUST), Pathogen Genomics Laboratory, Biological and Environmental Science and Engineering (BESE), Thuwal-Jeddah, 23955-6900, Saudi Arabia.ORCID 0000-0001-6601-3080
Qingtian GuanKing Abdullah University of Science and Technology (KAUST), Pathogen Genomics Laboratory, Biological and Environmental Science and Engineering (BESE), Thuwal-Jeddah, 23955-6900, Saudi Arabia.
Rahul P SalunkeKing Abdullah University of Science and Technology (KAUST), Pathogen Genomics Laboratory, Biological and Environmental Science and Engineering (BESE), Thuwal-Jeddah, 23955-6900, Saudi Arabia.
Amanda OoiKing Abdullah University of Science and Technology (KAUST), Pathogen Genomics Laboratory, Biological and Environmental Science and Engineering (BESE), Thuwal-Jeddah, 23955-6900, Saudi Arabia.
Luke EsauKing Abdullah University of Science and Technology (KAUST), Pathogen Genomics Laboratory, Biological and Environmental Science and Engineering (BESE), Thuwal-Jeddah, 23955-6900, Saudi Arabia.
Olga DouvropoulouKing Abdullah University of Science and Technology (KAUST), Pathogen Genomics Laboratory, Biological and Environmental Science and Engineering (BESE), Thuwal-Jeddah, 23955-6900, Saudi Arabia.
Raushan NugmanovaKing Abdullah University of Science and Technology (KAUST), Pathogen Genomics Laboratory, Biological and Environmental Science and Engineering (BESE), Thuwal-Jeddah, 23955-6900, Saudi Arabia.
Sadhasivam PerumalKing Abdullah University of Science and Technology (KAUST), Pathogen Genomics Laboratory, Biological and Environmental Science and Engineering (BESE), Thuwal-Jeddah, 23955-6900, Saudi Arabia.
Huoming ZhangKing Abdullah University of Science and Technology (KAUST), Pathogen Genomics Laboratory, Biological and Environmental Science and Engineering (BESE), Thuwal-Jeddah, 23955-6900, Saudi Arabia.ORCID 0000-0001-5416-0358
Issaac RajanKing Abdullah University of Science and Technology (KAUST), Pathogen Genomics Laboratory, Biological and Environmental Science and Engineering (BESE), Thuwal-Jeddah, 23955-6900, Saudi Arabia.
Awad Al-OmariDr. Suliman Al-Habib Medical Group, Riyadh, Saudi Arabia.
Samer SalihDr. Suliman Al-Habib Medical Group, Riyadh, Saudi Arabia.
Abbas ShamsanDr. Suliman Al-Habib Medical Group, Riyadh, Saudi Arabia.
Abbas Al MutairDr. Suliman Al-Habib Medical Group, Riyadh, Saudi Arabia.
Jumana TahaDepartment of Neuroscience, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia.
Abdulaziz AlahmadiDepartment of Preventive Medicine, Ministry of National Guard - Health Affairs, Riyadh, Saudi Arabia.
Nashwa KhotaniInfectious Diseases Medical Department, Al Noor Specialist Hospital Makkah, Makkah, MOH, Saudi Arabia.
Abdelrahman AlhamssGastroenterology Department, King Abdul Aziz Hospital Makkah, Makkah, MOH, Saudi Arabia.
Ahmed MahmoudCollege of Applied Medical Sciences, Taibah University, Madinah, Saudi Arabia.ORCID 0000-0002-1515-2352
Khaled AlquthamiInfectious Diseases Medical Department, Al Noor Specialist Hospital Makkah, Makkah, MOH, Saudi Arabia.
Abdullah DageegDepartment of Medicine, King Abdulaziz University Jeddah, Jeddah, Saudi Arabia.
Asim KhogeerPlan and Research Department, General Directorate of Health Affairs Makkah Region, Makkah, MOH, Saudi Arabia.
Anwar M HashemVaccines and Immunotherapy Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.ORCID 0000-0002-8471-7011
Paula MoragaKing Abdullah University of Science and Technology (KAUST), Computer, Electrical and Mathematical Science and Engineering Division (CEMSE), Thuwal-Jeddah, 23955-6900, Saudi Arabia.
Eric VolzSchool of Public Health, Faculty of Medicine, Imperial College, Norfolk Place, St Mary's Campus, London, United Kingdom.ORCID 0000-0001-6268-8937
Naif AlmontashiriCollege of Applied Medical Sciences, Taibah University, Madinah, Saudi Arabia.
Arnab PainKing Abdullah University of Science and Technology (KAUST), Pathogen Genomics Laboratory, Biological and Environmental Science and Engineering (BESE), Thuwal-Jeddah, 23955-6900, Saudi Arabia. arnab.pain@kaust.edu.sa.ORCID 0000-0002-1755-2819
King Abdullah University of Science and Technology · SAImperial College London · GBKing Abdulaziz University · SATaibah University · SAHera General Hospital · SAHokkaido University · JPKing Abdul Aziz University Hospital · SAKing Fahad Hospital Jeddah · SAKing Faisal Specialist Hospital & Research Centre · SAKing Saud bin Abdulaziz University for Health Sciences · SANational Guard Health Affairs · SA
Funding
King Abdulaziz City for Science and Technology (KACST) Proposal number: 5-20-01-002-0008King Abdullah University of Science and Technology (KAUST) KAUST faculty baseline fund (BAS/1/1020-01-01)King Abdullah University of Science and Technology (KAUST) Rapid Research Response Team (R3T) by Vice President - Research (VPR) office in KAUST.Medical Research Council MC_PC_19012Medical Research Council MR/R015600/1
Abstract
Monitoring SARS-CoV-2 spread and evolution through genome sequencing is essential in handling the COVID-19 pandemic. Here, we sequenced 892 SARS-CoV-2 genomes collected from patients in Saudi Arabia from March to August 2020. We show that two consecutive mutations (R203K/G204R) in the nucleocapsid (N) protein are associated with higher viral loads in COVID-19 patients. Our comparative biochemical analysis reveals that the mutant N protein displays enhanced viral RNA binding and differential interaction with key host proteins. We found increased interaction of GSK3A kinase simultaneously with hyper-phosphorylation of the adjacent serine site (S206) in the mutant N protein. Furthermore, the host cell transcriptome analysis suggests that the mutant N protein produces dysregulated interferon response genes. Here, we provide crucial information in linking the R203K/G204R mutations in the N protein to modulations of host-virus interactions and underline the potential of the nucleocapsid protein as a drug target during infection.
Indexed as
Genome, ViralMutation, MissenseCoronavirus Nucleocapsid ProteinsCOVID-19Glycogen Synthase Kinase 3Host-Pathogen InteractionsHumansNucleocapsidPhosphorylationPhylogenyProtein BindingSARS-CoV-2Saudi ArabiaViral LoadVirus ReplicationCoronavirus Nucleocapsid ProteinsGlycogen Synthase Kinase 3
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