ArticleNature communications2022
PplD is a de-N-acetylase of the cell wall linkage unit of streptococcal rhamnopolysaccharides.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Article
- Article
- A teichoic acid-like wall modification associated with immune suppression is socially regulated inmBio · 2026Article
- A conserved glycan motif induces broadly reactive functional antibodies against the zoonotic pathogenScience advances · 2026Article
- A small molecule PTER-selective inhibitor reduces food intake and body weight.bioRxiv : the preprint server for biology · 2026Article
- A teichoic acid-like wall modification associated with immune suppression is socially regulated inbioRxiv : the preprint server for biology · 2026Article
- Discovery of glycerol phosphate and an immunogenic glycan motif in rhamnose-rich polysaccharides of Streptococcus uberis.Veterinary research · 2025Article
- Natural variation of the streptococcal group A carbohydrate biosynthesis genes impacts host-pathogen interaction.Microbial genomics · 2025Article
- Glycosylation of serine/threonine-rich intrinsically disordered regions of membrane-associated proteins in streptococci.Nature communications · 2025Article
- Structure and mechanism of biosynthesis of Streptococcus mutans cell wall polysaccharide.Nature communications · 2025Article
- Recombinant production platform for Group A Streptococcus glycoconjugate vaccines.NPJ vaccines · 2025Article
- Variations of the NodB Architecture Are Attuned to Functional Specificities into and beyond the Carbohydrate Esterase Family 4.Biomolecules · 2024Article
- Human oral lectin ZG16B acts as a cell wall polysaccharide probe to decode host-microbe interactions with oral commensals.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Progress towards a glycoconjugate vaccine against Group A Streptococcus.NPJ vaccines · 2023Review
- Primary Structure of Glycans by NMR Spectroscopy.Chemical reviews · 2023Review
- The Cell Wall Deacetylases Spy1094 and Spy1370 Contribute toMicroorganisms · 2023Article
- Antibiotics and Carbohydrate-Containing Drugs Targeting Bacterial Cell Envelopes: An Overview.Pharmaceuticals (Basel, Switzerland) · 2022Review
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Authors and funding
15 authors at 5 institutions in 3 countries.
Funding
Abstract
The cell wall of the human bacterial pathogen Group A Streptococcus (GAS) consists of peptidoglycan decorated with the Lancefield group A carbohydrate (GAC). GAC is a promising target for the development of GAS vaccines. In this study, employing chemical, compositional, and NMR methods, we show that GAC is attached to peptidoglycan via glucosamine 1-phosphate. This structural feature makes the GAC-peptidoglycan linkage highly sensitive to cleavage by nitrous acid and resistant to mild acid conditions. Using this characteristic of the GAS cell wall, we identify PplD as a protein required for deacetylation of linkage N-acetylglucosamine (GlcNAc). X-ray structural analysis indicates that PplD performs catalysis via a modified acid/base mechanism. Genetic surveys in silico together with functional analysis indicate that PplD homologs deacetylate the polysaccharide linkage in many streptococcal species. We further demonstrate that introduction of positive charges to the cell wall by GlcNAc deacetylation protects GAS against host cationic antimicrobial proteins.
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