ArticleMethods in molecular biology (Clifton, N.J.)2022
Superresolution Microscopy for Visualization of Physical Contacts Between Chromosomes at Nanoscale Resolution.
Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- A working model for the formation of Robertsonian chromosomes.Journal of cell science · 2024Article
- Defining a core configuration for human centromeres during mitosis.Nature communications · 2023Article
- A hybrid RNA FISH immunofluorescence protocol on Drosophila polytene chromosomes.BMC research notes · 2023Article
- A hybrid RNA FISH immunofluorescence protocol onbioRxiv : the preprint server for biology · 2023Article
- Myc promotes polyploidy in murine trophoblast cells and suppresses senescence.Development (Cambridge, England) · 2023Article
- Defining a core configuration for human centromeres during mitosis.bioRxiv : the preprint server for biology · 2023Article
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Authors and funding
2 authors.
Funding
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Abstract
This protocol describes the fluorescence in situ hybridization (FISH) of DNA probes on mitotic chromosome spreads optimized for two super-resolution microscopy approaches-structured illumination microscopy (SIM) and stimulated emission depletion (STED). It is based on traditional DNA FISH methods that can be combined with immunofluorescence labeling (Immuno-FISH). This technique previously allowed us to visualize ribosomal DNA linkages between human acrocentric chromosomes and provided information about the activity status of linked rDNA loci. Compared to the conventional wide-field and confocal microscopy, the quality of SIM and STED data depends a lot more on the optimal specimen preparation, choice of fluorophores, and quality of the fluorescent labeling. This protocol highlights details that make specimens suitable for super-resolution microscopy and tips for good imaging practices.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.