ArticleAngewandte Chemie (International ed. in English)2022
Real-Time NMR Recording of Fermentation and Lipid Metabolism Processes in Live Microalgae Cells.
Article in Angewandte Chemie (International ed. in English), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Live Metabolomics with NMR.Analytical chemistry · 2026Review
- NMR Based Methods for Metabolites Analysis.Analytical chemistry · 2025Review
- Initial Primer Synthesis of a DNA Primase Monitored by Real-Time NMR Spectroscopy.Journal of the American Chemical Society · 2024Article
- Real-Time NMR Recording of Fermentation and Lipid Metabolism Processes in Live Microalgae Cells.Angewandte Chemie (International ed. in English) · 2022Article
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5 authors.
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Abstract
Non-invasive and real-time recording of processes in living cells has been limited to detection of small cellular components such as soluble proteins and metabolites. Here we report a multiphase NMR approach using magic-angle spinning NMR to synchronously follow microbial processes of fermentation, lipid metabolism and structural dynamic changes in live microalgae cells. Chlamydomonas reinhardtii green algae were highly concentrated, introducing dark fermentation and anoxia conditions. Single-pulse NMR experiments were applied to obtain temperature-dependent kinetic profiles of the formed fermentation products. Through dynamics-based spectral editing NMR, simultaneous conversion of galactolipids into TAG and free fatty acids was observed and rapid loss of rigid lipid structures. This suggests that lipolysis under dark and anoxia conditions finally results in the breakdown of cell and organelle membranes, which could be beneficial for recovery of intracellular microbial useful products.
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