ArticleJournal of biomedical optics2022
Using Fourier ptychography microscopy to achieve high-resolution chromosome imaging: an initial evaluation.
Article in Journal of biomedical optics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Fourier ptychography microscopy for digital pathology.Journal of microscopy · 2025Review
- VS-FPM: Large-Format, Label-Free Virtual Histopathology Microscopy.BME frontiers · 2025Article
- Development and Assessment of Multiple Illumination Color Fourier Ptychographic Microscopy for High Throughput Sample Digitization.Sensors (Basel, Switzerland) · 2024Article
- Peripheral Blood Leukocyte Detection Based on an Improved Detection Transformer Algorithm.Sensors (Basel, Switzerland) · 2023Article
- Using symmetric illumination and color camera to achieve high throughput Fourier ptychographic microscopy.Journal of biophotonics · 2023Article
- Optical ptychography for biomedical imaging: recent progress and future directions [Invited].Biomedical optics express · 2023Review
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Authors and funding
9 authors.
Funding
Abstract
significanceSearching analyzable metaphase chromosomes is a critical step for the diagnosis and treatment of leukemia patients, and the searching efficiency is limited by the difficulty that the conventional microscopic systems have in simultaneously achieving high resolution and a large field of view (FOV). However, this challenge can be addressed by Fourier ptychography microscopy (FPM) technology.
aimThe purpose of this study is to investigate the feasibility of utilizing FPM to reconstruct high-resolution chromosome images. APPROACH: An experimental FPM prototype, which was equipped with 4 × / 0.1 NA or 10 × / 0.25 NA objective lenses to achieve a theoretical equivalent NA of 0.48 and 0.63, respectively, was developed. Under these configurations, we first generated the system modulation transfer function (MTF) curves to assess the resolving power. Next, a group of analyzable metaphase chromosomes were imaged by the FPM system, which were acquired from the peripheral blood samples of the leukemia patients. The chromosome feature qualities were evaluated and compared with the results accomplished by the corresponding conventional microscopes.
resultsThe MTF curve results indicate that the resolving power of the 4 × / 0.1 NA FPM system is equivalent and comparable to the 20 × / 0.4 NA conventional microscope, whereas the performance of the 10 × / 0.25 NA FPM system is close to the 60 × / 0.95 NA conventional microscope. When imaging the chromosomes, the feature qualities of the 4 × / 0.1 NA FPM system are comparable to the results under the conventional 20 × / 0.4 NA lens, whereas the feature qualities of the 10 × / 0.25 NA FPM system are better than the conventional 60 × / 0.95 NA lens and comparable to the conventional 100 × / 1.25 NA lens.
conclusionsThis study initially verified that it is feasible to utilize FPM to develop a high-resolution and wide-field chromosome sample scanner.
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