ArticleThe plant genome2022
Chromosome-specific KASP markers for detecting Amblyopyrum muticum segments in wheat introgression lines.
Article in The plant genome, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- The transfer of 98% of the genome of Aegilops mutica into wheat (Triticum aestivum).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Investigations into genetic control of six spike traits with a focus on breeding for terminal heat stress tolerance in common wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- EasyKASP: a simple and fast tool for KASP primer design.BMC bioinformatics · 2025Article
- A simplified low-cost and reliable plant genomic DNA extraction method for PCR-based genotyping and screening.Plant methods · 2025Article
- Chromosome-level haplotype-resolved genome assembly of bread wheat's wild relative Aegilops mutica.Scientific data · 2025Article
- A segment ofFrontiers in plant science · 2025Article
- Genome-wide association and RNA-seq analyses reveal genes linked to salt stress in peanut (Frontiers in plant science · 2025Article
- Enrichment and Diversification of the Wheat Genome via Alien Introgression.Plants (Basel, Switzerland) · 2024Review
- Alien introgression to wheat for food security: functional and nutritional quality for novel products under climate change.Frontiers in nutrition · 2024Review
- The 4T and 7T introgressions fromFrontiers in plant science · 2024Article
- Effectiveness ofInternational journal of molecular sciences · 2023Article
- Whole-genome sequencing uncovers the structural and transcriptomic landscape of hexaploid wheat/Ambylopyrum muticum introgression lines.Plant biotechnology journal · 2023Article
- Genomic characterization and gene bank curation ofFrontiers in plant science · 2023Article
- Chromosome-specific KASP markers for detecting Amblyopyrum muticum segments in wheat introgression lines.The plant genome · 2022Article
- Validation of KASP markers associated with cassava mosaic disease resistance, storage root dry matter and provitamin A carotenoid contents in Ugandan cassava germplasm.Frontiers in plant science · 2022Article
- Introgression of theFrontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 3 countries.
Funding
Abstract
Many wild-relative species are being used in prebreeding programs to increase the genetic diversity of wheat (Triticum aestivum L.). Genotyping tools such as single nucleotide polymorphism (SNP)-based arrays and molecular markers have been widely used to characterize wheat-wild relative introgression lines. However, due to the polyploid nature of the recipient wheat genome, it is difficult to develop SNP-based Kompetitive allele-specific polymerase chain reaction (KASP) markers that are codominant to track the introgressions from the wild species. Previous attempts to develop KASP markers have involved both exome- and polymerase chain reaction (PCR)-amplicon-based sequencing of the wild species. But chromosome-specific KASP assays have been hindered by homoeologous SNPs within the wheat genome. This study involved whole genome sequencing of the diploid wheat wild relative Amblyopyrum muticum (Boiss.) Eig and development of a de novo SNP discovery pipeline that generated ∼38,000 SNPs in unique wheat genome sequences. New assays were designed to increase the density of Am. muticum polymorphic KASP markers. With a goal of one marker per 60 Mbp, 335 new KASP assays were validated as diagnostic for Am. muticum in a wheat background. Together with assays validated in previous studies, 498 well distributed chromosome-specific markers were used to recharacterize previously genotyped wheat-Am. muticum doubled haploid (DH) introgression lines. The chromosome-specific nature of the KASP markers allowed clarification of which wheat chromosomes were involved with recombination events or substituted with Am. muticum chromosomes and the higher density of markers allowed detection of new small introgressions in these DH lines.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.