ArticleAging cell2022
Loss of circRNAs from the crh-1 gene extends the mean lifespan in Caenorhabditis elegans.
Article in Aging cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- CircRNA-regulated programmed cell death networks in cardiomyocytes: Molecular crosstalk and therapeutic translation.Non-coding RNA research · 2026Review
- The RNA-binding protein NOVA-1 regulates circRNA expression, alternative splicing, and aging in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2026Article
- Review
- Circular RNAs exhibit exceptional stability in the aging brain and serve as reliable age and experience indicators.Cell reports · 2025Article
- Loss of age-accumulatedFrontiers in aging neuroscience · 2025Article
- Recent progress in regulation of aging by non-coding RNAs inAnimal cells and systems · 2025Review
- A brain-enriched circular RNA controls excitatory neurotransmission and restricts sensitivity to aversive stimuli.Science advances · 2024Article
- Non-coding RNA in the gut of the blood-feeding parasitic worm, Haemonchus contortus.Veterinary research · 2024Article
- Regulation of alternative splicing and polyadenylation in neurons.Life science alliance · 2023Review
- Circular RNAs in the Origin of Developmental Lung Disease: Promising Diagnostic and Therapeutic Biomarkers.Biomolecules · 2023Review
- Loss of circRNAs from the crh-1 gene extends the mean lifespan in Caenorhabditis elegans.Aging cell · 2022Article
- Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Accumulation of circular RNAs (circRNAs) during aging occurs on a genome-wide level for multiple organisms, but its significance is unknown. Generating circRNA loss-of-function mutants is difficult because the vast majority of these RNAs are comprised of exons shared with protein-coding mRNAs. In Caenorhabditis elegans, most circRNAs were previously found to accumulate during aging. Two of the most abundant, age-accumulating circRNAs are generated from exon 4 of the crh-1 gene (circ-crh-1). Here, we found that the biogenesis of circ-crh-1 was regulated by the double-stranded RNA-binding protein ADR-1. We identified Reverse Complementary Match (RCM) sequences in introns flanking circ-crh-1. Using CRISPR-Cas9, we deleted the downstream RCM and found that this completely eliminated expression of the circRNA without affecting linear mRNA expression from the crh-1 gene. Remarkably, worms lacking circ-crh-1 exhibited a significantly longer mean lifespan. Lifespan was partially restored to wild type by expression of circ-crh-1 in neural tissues. Widespread transcriptome alterations in circ-crh-1 mutants were identified using RNA-Seq. Moving forward, intronic RCM deletion using CRISPR should be a widely applicable method to identify lifespan-regulating circRNAs in C. elegans.
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