ArticleNature biotechnology2022
Computationally designed dual-color MRI reporters for noninvasive imaging of transgene expression.
Article in Nature biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Chiral multimodal nanoprobes for sensing of biomarkers.Nature protocols · 2026Review
- A genetic reporter for visualizing nitroreductase activity using magnetic resonance imaging.Chemistry methods : new approaches to solving problems in chemistry · 2026Article
- Noninvasive Imaging of Transgene Expression in Neurons Using Chemical Exchange Saturation Transfer MRI.NMR in biomedicine · 2025Article
- A Genetically Engineered Reporter System Designed forJournal of the American Chemical Society · 2024Article
- Opportunities and challenges in design and optimization of protein function.Nature reviews. Molecular cell biology · 2024Review
- Destabilized reporters for background-subtracted, chemically-gated, and multiplexed deep-tissue imaging.Chemical science · 2024Article
- Photoacoustic Tomography with Temporal Encoding Reconstruction (PATTERN) for cross-modal individual analysis of the whole brain.Nature communications · 2024Article
- A Dual-Gene Reporter-Amplifier Architecture for Enhancing the Sensitivity of Molecular MRI by Water Exchange.Chembiochem : a European journal of chemical biology · 2024Article
- Engineering water exchange is a safe and effective method for magnetic resonance imaging in diverse cell types.Journal of biological engineering · 2024Article
- A dual-gene reporter-amplifier architecture for enhancing the sensitivity of molecular MRI by water exchange.bioRxiv : the preprint server for biology · 2024Article
- Theranostics - a sure cure for cancer after 100 years?Theranostics · 2024Review
- A putative design for the electromagnetic activation of split proteins for molecular and cellular manipulation.Frontiers in bioengineering and biotechnology · 2024Article
- Engineering water exchange is a safe and effective method for magnetic resonance imaging in diverse cell types.bioRxiv : the preprint server for biology · 2023Article
- Development of a synthetic biosensor for chemical exchange MRI utilizing in silico optimized peptides.NMR in biomedicine · 2023Article
- Genomically mined acoustic reporter genes for real-time in vivo monitoring of tumors and tumor-homing bacteria.Nature biotechnology · 2023Article
- Protein and peptide engineering for chemical exchange saturation transfer imaging in the age of synthetic biology.NMR in biomedicine · 2023Review
- A FRET-Based Ratiometric HResearch (Washington, D.C.) · 2023Article
- Designing Protein-Based Probes for Sensing Biological Analytes with Magnetic Resonance Imaging.Analysis & sensing · 2022Article
- Reporter Genes for Brain Imaging Using MRI, SPECT and PET.International journal of molecular sciences · 2022Review
- What Have We Learned from Design of Function in Large Proteins?Biodesign research · 2022Review
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Imaging of gene-expression patterns in live animals is difficult to achieve with fluorescent proteins because tissues are opaque to visible light. Imaging of transgene expression with magnetic resonance imaging (MRI), which penetrates to deep tissues, has been limited by single reporter visualization capabilities. Moreover, the low-throughput capacity of MRI limits large-scale mutagenesis strategies to improve existing reporters. Here we develop an MRI system, called GeneREFORM, comprising orthogonal reporters for two-color imaging of transgene expression in deep tissues. Starting from two promiscuous deoxyribonucleoside kinases, we computationally designed highly active, orthogonal enzymes ('reporter genes') that specifically phosphorylate two MRI-detectable synthetic deoxyribonucleosides ('reporter probes'). Systemically administered reporter probes exclusively accumulate in cells expressing the designed reporter genes, and their distribution is displayed as pseudo-colored MRI maps based on dynamic proton exchange for noninvasive visualization of transgene expression. We envision that future extensions of GeneREFORM will pave the way to multiplexed deep-tissue mapping of gene expression in live animals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.