ArticleScientific reports2022
In vivo melanin 3D quantification and z-epidermal distribution by multiphoton FLIM, phasor and Pseudo-FLIM analyses.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 27 citations in OpenAlex.
- Decoding melanin dynamics: Photoacoustic microscopy reveals differential aging of metabolic and keratinized skin zones.Photoacoustics · 2026Article
- Bacterial porphyrins in healthy skin: Microbiota components impact melanogenesis and age-related processes leading to Porphyr'ageing.International journal of cosmetic science · 2026Article
- QUILPEN Provides Independent and Label-Free Single-Cell Quantification of Pigmentation Dynamics and Organelle Content.Pigment cell & melanoma research · 2026Article
- AI-assisted quantitative analysis for evaluating melanin distribution in 3D pigmented epidermis-on-a-chip models.Frontiers in bioengineering and biotechnology · 2026Article
- Label-free human skin imaging with enhanced molecular contrast via time-resolved fluorescence and advanced phasor analysis.Communications biology · 2025Article
- A Non-Invasive Approach to Intracellular Measurement in Solar Lentigo: Investigating Mitochondrial Dysfunction and Senescence Mechanisms Associated with Excessive Melanin Deposition.International journal of molecular sciences · 2025Article
- Label-free multiphoton microscopy of human skin: longitudinal studies capturing cell dynamics.Biomedical optics express · 2025Article
- Impact of skin tone on target size detectability in photoacoustic breast imaging.Biophotonics discovery · 2024Article
- Comparison of facial skin ageing in healthy Asian and Caucasian females quantified by in vivo line-field confocal optical coherence tomography 3D imaging.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2024Article
- Melanometry for objective evaluation of skin pigmentation in pulse oximetry studies.Communications medicine · 2024Review
- Mitigating skin tone bias in linear arrayPhotoacoustics · 2023Article
- Optical Methods for Non-Invasive Determination of Skin Penetration: Current Trends, Advances, Possibilities, Prospects, and Translation into In Vivo Human Studies.Pharmaceutics · 2023Review
- Multiphoton FLIM Analyses of Native and UVA-Modified Synthetic Melanins.International journal of molecular sciences · 2023Article
- In vivo multiphoton multiparametric 3D quantification of human skin aging on forearm and face.Scientific reports · 2022Article
- A Criterion of Colorectal Cancer Diagnosis Using Exosome Fluorescence-Lifetime Imaging.Diagnostics (Basel, Switzerland) · 2022Article
- In vivo imaging with a fast large-area multiphoton exoscope (FLAME) captures the melanin distribution heterogeneity in human skin.Scientific reports · 2022Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Characterizing melanins in situ and determining their 3D z-epidermal distribution is paramount for understanding physiological/pathological processes of melanin neosynthesis, transfer, degradation or modulation with external UV exposure or cosmetic/pharmaceutical products. Multiphoton fluorescence intensity- and lifetime-based approaches have been shown to afford melanin detection, but how can one quantify melanin in vivo in 3D from multiphoton fluorescence lifetime (FLIM) data, especially since FLIM imaging requires long image acquisition times not compatible with 3D imaging in a clinical setup? We propose an approach combining (i) multiphoton FLIM, (ii) fast image acquisition times, and (iii) a melanin detection method called Pseudo-FLIM, based on slope analysis of autofluorescence intensity decays from temporally binned data. We compare Pseudo-FLIM to FLIM bi-exponential and phasor analyses of synthetic melanin, melanocytes/keratinocytes coculture and in vivo human skin. Using parameters of global 3D epidermal melanin density and z-epidermal distribution profile, we provide first insights into the in vivo knowledge of 3D melanin modulations with constitutive pigmentation versus ethnicity, with seasonality over 1 year and with topical application of retinoic acid or retinol on human skin. Applications of Pseudo-FLIM based melanin detection encompass physiological, pathological, or environmental factors-induced pigmentation modulations up to whitening, anti-photoaging, or photoprotection products evaluation.
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