Evidence map›Paper›PMID 35101743›Full record

ArticleBiomaterials2022

Rapid 3D bioprinting of a multicellular model recapitulating pterygium microenvironment.

Zheng Zhong, Jing Wang, Jing Tian, Xiaoqian Deng, Alis Balayan, Yazhi Sun, Yi Xiang, Jiaao Guan, Jacob Schimelman, Henry Hwang and 7 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed, 1 pooled it
3.9field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Review
  9. Review
  10. Engineering Heterogeneous Tumor Models for Biomedical Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
    Review
  11. Focus on seed cells: stem cells in 3D bioprinting of corneal grafts.Frontiers in bioengineering and biotechnology · 2024
    Review
  12. Review
  13. Article
  14. Article
  15. Light-based vat-polymerization bioprinting.Nature reviews. Methods primers · 2023
    Article
  16. Three-dimensional bioprinting in ophthalmic care.International journal of ophthalmology · 2023
    Review
  17. Article
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 2 institutions in 1 country.

Zheng ZhongDepartment of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA.
Jing WangDepartment of Human Genetics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, 90095, USA.
Jing TianDepartment of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA.
Xiaoqian DengDepartment of Human Genetics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, 90095, USA.
Alis BalayanDepartment of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA; School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Yazhi SunDepartment of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA.
Yi XiangDepartment of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA.
Jiaao GuanDepartment of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA.
Jacob SchimelmanDepartment of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA.
Henry HwangDepartment of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA.
Shangting YouDepartment of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA.
Xiaokang WuSchool of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Chao MaStein Eye Institute, University of California Los Angeles, Los Angeles, CA, 90095, USA.
Xiaoao ShiDepartment of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA.
Emmie YaoDepartment of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA.
Sophie X DengStein Eye Institute, University of California Los Angeles, Los Angeles, CA, 90095, USA.
Shaochen ChenDepartment of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA. Electronic address: chen168@eng.ucsd.edu.
University of California San Diego · USUniversity of California, Los Angeles · US

Funding

U of Calif, San Diego Neuroscience Microscopy ImagingP30NS047101 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GLEESON, JOSEPH G, ZHENG, BINHAI · 2003 to 2022
$9.0M
Rapid 3D bioprinting of biomimetic vascularized tissue constructsR01EB021857 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHEN, SHAOCHEN, DOKMECI, MEHMET REMZI · 2016 to 2019
$2.1M
A stem cell based optic nerve model for studies of axon guidance and regenerationR21EY031122 · NEI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHEN, SHAOCHEN, WAHLIN, KARL J · 2019 to 2019
$299k
NEI NIH HHS R21 EY031122NIBIB NIH HHS R01 EB021857NINDS NIH HHS P30 NS047101
6 · The paper itself

Abstract

Pterygium is an ocular surface disorder with high prevalence that can lead to vision impairment. As a pathological outgrowth of conjunctiva, pterygium involves neovascularization and chronic inflammation. Here, we developed a 3D multicellular in vitro pterygium model using a digital light processing (DLP)-based 3D bioprinting platform with human conjunctival stem cells (hCjSCs). A novel feeder-free culture system was adopted and efficiently expanded the primary hCjSCs with homogeneity, stemness and differentiation potency. The DLP-based 3D bioprinting method was able to fabricate hydrogel scaffolds that support the viability and biological integrity of the encapsulated hCjSCs. The bioprinted 3D pterygium model consisted of hCjSCs, immune cells, and vascular cells to recapitulate the disease microenvironment. Transcriptomic analysis using RNA sequencing (RNA-seq) identified a distinct profile correlated to inflammation response, angiogenesis, and epithelial mesenchymal transition in the bioprinted 3D pterygium model. In addition, the pterygium signatures and disease relevance of the bioprinted model were validated with the public RNA-seq data from patient-derived pterygium tissues. By integrating the stem cell technology with 3D bioprinting, this is the first reported 3D in vitro disease model for pterygium that can be utilized for future studies towards personalized medicine and drug screening.

Indexed as

BioprintingPterygiumConjunctivaHumansHydrogelsInflammationPrinting, Three-DimensionalTissue EngineeringTissue ScaffoldsHydrogels3D bioprintingDisease modelEpithelial mesenchymal transitionHydrogelsPterygiumStem cellsTissue engineering

Identifiers

PMID35101743
PMCPMC10162446
OpenAlexW4210554875

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.