Evidence map›Paper›PMID 35099735›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

An Ex Vivo Tissue Culture Method for Discovering Cell Dynamics Involved in Stromal Vascular Fraction Vasculogenesis Using the Mouse Mesentery.

Dima Majbour, Ariana D Suarez-Martinez, Nicholas A Hodges, Arinola O Lampejo, Banks M Lomel, Elijah W Rice, Hulan Shang, Adam J Katz, Walter L Murfee

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.0field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Dima MajbourCrayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
Ariana D Suarez-MartinezCrayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
Nicholas A HodgesCrayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
Arinola O LampejoCrayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
Banks M LomelCrayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
Elijah W RiceDepartment of Plastic and Reconstructive Surgery, Wake Forest School of Medicine, Winston-Salem, NC, USA.
Hulan ShangCrayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
Adam J KatzCrayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
Walter L MurfeeCrayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA. wmurfee@bme.ufl.edu.
University of Florida · USWake Forest University · US

Funding

Angiogenesis Model for Aging ResearchR01AG049821 · NIA · UNIVERSITY OF FLORIDA · PI MURFEE, WALTER L · 2016 to 2019
$1.0M
NIA NIH HHS R01 AG049821
6 · The paper itself

Abstract

Stromal vascular fraction (SVF), isolated from adipose tissue, identifies as a rich cell source comprised of endothelial cells, endothelial progenitor cells, pericytes, smooth muscle cells, fibroblasts, and immune cells. SVF represents a promising therapeutic heterogonous cell source for growing new blood microvessels due to its rich niche of cells. However, the spatiotemporal dynamics of SVF within living tissues remain largely unknown. The objective of this chapter is to describe a protocol for culturing SVF on mouse mesentery tissues in order to aid in the discovery of SVF dynamics and associated vessel growth over time. SVF was isolated from the inguinal adipose from adult mice and seeded onto mesentery tissues. Tissues were then cultured for up to 5 days and labeled with endothelial cell and pericyte markers. Representative results demonstrate the observation of SVF-derived vasculogenesis characterized by de novo vessel formation and subsequent vessel connection.

Indexed as

Endothelial CellsStromal CellsAdipose TissueAnimalsCells, CulturedMesenteryMiceStromal Vascular FractionAngiogenesisMesenteryMicrocirculationStromal Vascular FractionTissue CultureVasculogenesis

Identifiers

PMID35099735
OpenAlexW4210789931

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.