Evidence map›Paper›PMID 35099564›Full record

ArticlePlant physiology2022

Maize Interveinal Chlorosis 1 links the Yang Cycle and Fe homeostasis through Nicotianamine biosynthesis.

Wentao Sun, Xiaojin J Zhou, Chen Chen, Xin Zhang, Xiaolong Tian, Ke Xiao, Chenxu Liu, Rumei Chen, Shaojiang Chen

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.3field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 7 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Wentao SunNational Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100194, China.
Xiaojin J ZhouCrop Functional Genomics Center, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.ORCID 0000-0002-9309-4936
Chen ChenNational Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100194, China.
Xin ZhangCrop Functional Genomics Center, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Xiaolong TianNational Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100194, China.ORCID 0000-0003-1993-7605
Ke XiaoCrop Functional Genomics Center, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Chenxu LiuNational Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100194, China.ORCID 0000-0003-0618-881X
Rumei ChenCrop Functional Genomics Center, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.ORCID 0000-0003-4449-3081
Shaojiang ChenNational Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100194, China.ORCID 0000-0002-7138-5222
China Agricultural University · CNChinese Academy of Agricultural Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Yang cycle is involved in many essential metabolic pathways in plant growth and development. As extended products of the Yang cycle, the function and regulation network of ethylene and polyamines are well characterized. Nicotianamine (NA) is also a product of this cycle and works as a key metal chelator for iron (Fe) homeostasis in plants. However, interactions between the Yang cycle and NA biosynthesis remain unclear. Here, we cloned maize interveinal chlorosis 1 (mic1), encoding a 5'-methylthioadenosine nucleosidase (MTN), that is essential for 5'-methylthioadenosine (MTA) salvage and NA biosynthesis in maize (Zea mays). A single base G-A transition in the fourth exon of mic1 causes a Gly to Asp change, resulting in increased MTA, reduced Fe distribution, and growth retardation of seedlings. Knockout of ZmMIC1 but not its paralog ZmMTN2 by CRISPR/Cas9 causes interveinal chlorosis, indicating ZmMIC1 is mainly responsible for MTN activity in maize. Transcriptome analysis showed a typical response of Fe deficiency. However, metabolic analysis revealed dramatically reduced NA content in mic1, suggesting NA biosynthesis was impaired in the mutant. Exogenous application of NA transiently reversed the interveinal chlorosis phenotype of mic1 seedlings. Moreover, the mic1 mutant overexpressing a NA synthase gene not only recovered from interveinal chlorosis and growth retardation but was also fertile. These findings provide a link between the Yang cycle and NA biosynthesis, which highlights an aspect of Fe homeostasis regulation in maize.

Indexed as

Anemia, HypochromicZea maysAzetidinecarboxylic AcidGene Expression Regulation, PlantHomeostasisAzetidinecarboxylic Acidnicotianamine

Identifiers

PMID35099564
PMCPMC8968279
OpenAlexW4210683320

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.