ArticleJournal of assisted reproduction and genetics2022
Comparison between slow freezing and vitrification of ovarian tissue cryopreservation in assigned female at birth transgender people receiving testosterone therapy: data on histological and viability parameters.
Article in Journal of assisted reproduction and genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 3 syntheses or guidelines pooled it, 12 citations in OpenAlex.
- Comparison of the quality of ovarian tissue cryopreservation by conventional slow cryopreservation and vitrification-a systematic review and meta-analysis.Journal of ovarian research · 2025Pooled it
- Vitrification versus slow freezing of human ovarian tissue: a systematic review and meta-analysis of histological outcomes.Journal of assisted reproduction and genetics · 2023Pooled it
- SART CORS IVF registry: looking to the past to shape future perspectives.Journal of assisted reproduction and genetics · 2022Pooled it
- Safety assessment of autologous ovarian tissue cryopreservation and transplantation in cancer patients: an updated review.Journal of ovarian research · 2026Review
- Comparison of the biomechanical characteristics of human ovarian tissue after vitrification versus slow freezing.JBRA assisted reproduction · 2025Article
- The walnut-derived peptide TW-7 improves mouse parthenogenetic embryo development of vitrified MII oocytes potentially by promoting histone lactylation.Journal of animal science and biotechnology · 2024Article
- Evaluation of heat transfer in porous scaffolds under cryogenic treatment: a numerical study.Medical & biological engineering & computing · 2023Article
- Article
- "Lights and Shades" of Fertility Preservation in Transgender Men Patients: A Clinical and Pathological Review.Life (Basel, Switzerland) · 2023Review
- Barriers to fertility preservation access in transgender and gender diverse adolescents: a narrative review.Therapeutic advances in reproductive healthReview
- First Childbirth after Fertility Preservation and Gender Reassigning Surgery from Indian Transgender Community.Journal of human reproductive sciencesArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe use of fertility preservation (FP) techniques has significantly increased in recent years in the assigned female at birth (AFAB) transgender population. Oocyte cryopreservation is the established method for FP, but ovarian tissue cryopreservation may be considered an alternative option, especially during gender-affirming surgery (GAS). The slow freezing (SF) cryopreservation technique is the standard method for human ovarian tissue, but recently, several studies have shown good results with the vitrification (VT) technique. The objective of this study was to compare the effectiveness of VT and SF techniques in ovarian tissue from AFAB transgender people.
methodsThis was a prospective study including 18 AFAB transgender people after GAS. Ovarian tissue pieces from each ovary were cryopreserved by SF and VT and compared with fresh tissue. Study by light microscopy (LM) assessed follicular morphology and density. The percentage of surviving and degenerated follicles was studied with the tissue viability test. Oocytes, granulosa cells and stroma were analysed separately by transmission electron microscopy.
resultsThe VT technique preserves follicle and stromal tissue as well as the SF method, but with some differences. Evaluation by LM showed better follicle preservation with VT, but the ultrastructural study showed the presence of minor damage with both techniques compared to fresh tissue.
conclusionBoth cryopreservation techniques are accurate for maintaining the follicular population and stromal tissue. Further studies are needed to determine the impact of VT on ovarian tissue and the subsequent follicular activation mechanisms in AFAB ovarian tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.