Evidence map›Paper›PMID 35098395›Full record

ArticleMolecular biology reports2022

Modified uvsY by N-terminal hexahistidine tag addition enhances efficiency of recombinase polymerase amplification to detect SARS-CoV-2 DNA.

Kevin Maafu Juma, Teisuke Takita, Masaya Yamagata, Mika Ishitani, Kaichi Hayashi, Kenji Kojima, Koichiro Suzuki, Yuri Ando, Wakao Fukuda, Shinsuke Fujiwara and 3 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 1 country.

Kevin Maafu JumaDivision of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.
Teisuke TakitaDivision of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.
Masaya YamagataDivision of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.
Mika IshitaniDivision of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.
Kaichi HayashiDivision of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.
Kenji KojimaDivision of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.
Koichiro SuzukiThe Research Foundation for Microbial Diseases of Osaka University, Suita, Osaka, 565-0871, Japan.
Yuri AndoDepartment of Biosciences, School of Biological and Environmental Sciences, Kwansei-Gakuin University, Sanda, Hyogo, 669‑1337, Japan.
Wakao FukudaDepartment of Biosciences, School of Biological and Environmental Sciences, Kwansei-Gakuin University, Sanda, Hyogo, 669‑1337, Japan.
Shinsuke FujiwaraDepartment of Biosciences, School of Biological and Environmental Sciences, Kwansei-Gakuin University, Sanda, Hyogo, 669‑1337, Japan.
Yukiko NakuraDepartment of Developmental Medicine, Research Institute, Osaka Women's and Children's Hospital, Izumi-shi, Osaka, 594-1101, Japan.
Itaru YanagiharaDepartment of Developmental Medicine, Research Institute, Osaka Women's and Children's Hospital, Izumi-shi, Osaka, 594-1101, Japan.
Kiyoshi YasukawaDivision of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan. yasukawa.kiyoshi.7v@kyoto-u.ac.jp.ORCID http://orcid.org/0000-0001-7748-6043
Kyoto University · JPKwansei Gakuin University · JPOsaka Women's and Children's Hospital · JPHimeji Dokkyo University · JPThe University of Osaka · JP

Funding

Japan Agency for Medical Research and Development 20fk0108143h001Japan Agency for Medical Research and Development 20he0622020h0001Japan Science and Technology Agency JPMJTR20UUJapan Society for the Promotion of Science 18KK0285
6 · The paper itself

Abstract

backgroundRecombinase (uvsY and uvsX) from bacteriophage T4 is a key enzyme for recombinase polymerase amplification (RPA) that amplifies a target DNA sequence at a constant temperature with a single-stranded DNA-binding protein and a strand-displacing polymerase. The present study was conducted to examine the effects of the N- and C-terminal tags of uvsY on its function in RPA to detect SARS-CoV-2 DNA.

methodsUntagged uvsY (uvsY-Δhis), N-terminal tagged uvsY (uvsY-Nhis), C-terminal tagged uvsY (uvsY-Chis), and N- and C-terminal tagged uvsY (uvsY-NChis) were expressed in Escherichia coli and purified. RPA reaction was carried out with the in vitro synthesized standard DNA at 41 °C. The amplified products were separated on agarose gels.

resultsThe minimal initial copy numbers of standard DNA from which the amplified products were observed were 6 × 10

conclusionsThe reaction efficiency of RPA with uvsY-Nhis was the highest, suggesting that uvsY-Nhis is suitable for use in RPA.

Indexed as

Nucleic Acid Amplification TechniquesBacteriophage T4DNA-Binding ProteinsDNA, ViralMembrane ProteinsSARS-CoV-2Viral ProteinsDNA-Binding ProteinsDNA, ViralMembrane ProteinsUvsX protein, Enterobacteria phage T4UvsY protein, Enterobacteria phage T4Viral ProteinsHexahistidine tagIsothermal DNA amplificationRecombinase polymerase amplification (RPA)uvsY

Identifiers

PMID35098395
PMCPMC8801280
OpenAlexW4220763465

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.