Evidence map›Paper›PMID 35098163›Full record

ArticleSTAR protocols2022

Tracking the dynamics of single fused synaptic vesicle proteins from a single ribbon active zone in zebrafish retinal bipolar cells.

Abhishek P Shrestha, Thirumalini Vaithianathan

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.2field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Abhishek P ShresthaDepartment of Pharmacology, Addiction Science, and Toxicology, University of Tennessee Health Science Center, Memphis, TN, USA.
Thirumalini VaithianathanDepartment of Pharmacology, Addiction Science, and Toxicology, University of Tennessee Health Science Center, Memphis, TN, USA.
University of Tennessee Health Science Center · US

Funding

Dynamics of calcium signals control neurotransmitter release in retinal ribbon synapses.R01EY030863 · NEI · UNIVERSITY OF TENNESSEE HEALTH SCI CTR · PI VAITHIANATHAN, THIRUMALINI · 2021 to 2025
$2.3M
NEI NIH HHS R01 EY030863
6 · The paper itself

Abstract

Clearance of fused synaptic vesicle components and availability of release sites are important determinants of recovery from short-term synaptic depression. However, the dynamics of release site clearance are not well established. This protocol illustrates single-molecule imaging of an exocytosis reporter, synaptophysin-pHluorin fusion protein (SypHy), by combining two-color laser scanning confocal microscopy with whole-cell patch-clamp recording of retinal bipolar cells from transgenic zebrafish that weakly express SypHy to track the dynamics of newly fused vesicle proteins at the active zone. For complete details on the use and execution of this profile, please refer to Vaithianathan et al. (2019).

Indexed as

AnimalsExocytosisNerve Tissue ProteinsPatch-Clamp TechniquesRetinaSynaptic VesiclesZebrafishNerve Tissue ProteinsCell BiologyMicroscopyModel OrganismsMolecular/Chemical ProbesNeuroscience

Identifiers

PMID35098163
PMCPMC8783205
OpenAlexW4206311966

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.